The spot orientation must be one of the following:

  1. Horizontal Left to Right, Vertical Top to Bottom, Spot 2 horizontal of Spot 1
  2. Horizontal Left to Right, Vertical Bottom to Top, Spot 2 horizontal of Spot 1
  3. Horizontal Right to Left, Vertical Top to Bottom, Spot 2 horizontal of Spot 1
  4. Horizontal Right to Left, Vertical Bottom to Top, Spot 2 horizontal of Spot 1

It is not possible for Spot 2 to be vertical of Spot 1, because all spots in Row 1 are listed before the first spot in Row 2.

See MAGIC Tool Addressing tutorial for illustrations of each of the eight possible spot orientations.

Bioinformatics Genomics MAGIC Tool Genomics GCAT

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