LOCUS             pLac_GFP_1C3        2877 bp ds-DNA   circular     02-AUG-2011
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     RBS             2136..2147
                     /label=B0034 (RBS)
                     /ApEinfo_fwdcolor=#3300cc
                     /ApEinfo_revcolor=#6633ff
     misc_feature    22..56
                     /label=pSB3K3 Post-Suffix
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#336666
     promoter        2072..2126
                     /label=R0011 (pLac)
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
     misc_feature    2016..2050
                     /label=3k3 Pre-prefix
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#336666
     misc_feature    join(2091..2096,2114..2119)
                     /label=RNAp binding motif (17bp)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    709..714
                     /label=XbaI/SpeI Scar
                     /ApEinfo_fwdcolor=#cc9999
                     /ApEinfo_revcolor=#ffcccc
     misc_binding    2096..2114
                     /label=lac1 core operator
                     /ApEinfo_fwdcolor=#3469ff
                     /ApEinfo_revcolor=#00ffff
     primer_bind     complement(157..176)
                     /label=Vr (biobrick primer)
                     /ApEinfo_fwdcolor=#9900cc
                     /ApEinfo_revcolor=#cc33ff
     misc_binding    2072..2092
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
     primer_bind     1933..1952
                     /label=Vf2 (biobrick primer)
                     /ApEinfo_fwdcolor=#9900cc
                     /ApEinfo_revcolor=#cc33ff
     RBS             2136..2147
                     /label=RBS (b0034)
                     /ApEinfo_fwdcolor=#0000a0
                     /ApEinfo_revcolor=green
     misc_binding    2051..2071
                     /label=bglBrk Prefix
                     /ApEinfo_fwdcolor=#3469ff
                     /ApEinfo_revcolor=#00ffff
     misc_signal     2334..2351
                     /label=RNase match in GFP
                     /ApEinfo_fwdcolor=#e1031e
                     /ApEinfo_revcolor=green
     misc_binding    1..21
                     /label=bglBrk Suffix
                     /ApEinfo_fwdcolor=#3469ff
                     /ApEinfo_revcolor=#00ffff
     misc_signal     complement(2334..2351)
                     /label=Match to RNase
                     /ApEinfo_fwdcolor=#e1031e
                     /ApEinfo_revcolor=green
     CDS             2164..2877
                     /label=GFP (E0040) no stop
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=green
ORIGIN
        1 GGATCCAACA GGGTTCTCGA Gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1141 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1201 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1261 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1321 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1381 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1441 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1501 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1561 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1621 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1681 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1741 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1801 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1861 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1921 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     1981 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2041 atgatttctg GAATTCCTGC TGCGGAGATC TAATTGTGAG CGGATAACAA TTGACATTGT
     2101 GAGCGGATAA CAAGATACTG AGCACAGAAT TCATTAAAGA GGAGAAAAAA AAAAAAAAAA
     2161 AAAATGCGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT
     2221 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC
     2281 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGCCAACA
     2341 CTTGTCACTA CTTTCGGTTA TGGTGTTCAA TGCTTTGCGA GATACCCAGA TCATATGAAA
     2401 CAGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT
     2461 TTCAAAGATG ACGGGAACTA CAAGACACGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT
     2521 GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC
     2581 AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT
     2641 GGAATCAAAG TTAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA
     2701 GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT
     2761 TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC
     2821 CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAA
//