View source for Divide and Conquer Biological Challenges

<center>[[Image:iGEM2008_design.jpg]]</center>

=== * We need to get the Lux/AHL system working. ===
=== * We need to get the Lsr/AI-2 system working. ===
'''Our summary of the Lsr system:''' [[Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system]]<br>
* There are '''NO''' Lsr parts in the registry: <br>
*We will need to make BB parts for:<br>
# LsrR and LsrK which are adjacent to each other in the E. coli genome. [http://www.ncbi.nlm.nih.gov/projects/sviewer/?id=NC_010473.1&v=1687890..1688937 We could amplify them together with a total size of over 2600bp.] 
# Lsr promoter
# LuxS that produces DPD that somehow is converted to ''R''-THMF.
# How do we get cells to make AI-2?

=== * We do NOT need to use additional chemical input signals (''e.g.'', aTc and IPTG). We will use only AHL and AI-2 added exogenously. ===

=== * We need to have an XOR logic gate produced. ===
'''Existing Logic Gates'''
*NOT: (insert links to examples here)
*AND: (insert links to examples here)
=== * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon. ===
What if Amp<sup>R</sup> is secreted and cells are not Amp<sup>R</sup>? This would prevent cells down the chain from responding too soon.