View source for IGEM 2009 Project

Biology Based:
#[[Which reporters are we going to use?]]
#[[What naming system are we going to use for the suppressor tRNAs?]]
#[[How do you build the tRNA construct?]]
#[[How are we going to build the 5-mer BioBricks?]]

[http://gcat.davidson.edu/GcatWiki/images/2/2c/Stop_Codons_in_LCs.doc Using Stop Codons to Truncate Translation]<br>
[http://gcat.davidson.edu/GcatWiki/images/8/81/D5merSequences.doc Davidson ATG+5mer BioBrick Sequences]

[http://gcat.davidson.edu/GcatWiki/images/4/47/FML_CODING_SEQUENCE_2.doc Frameshift Mutation Leader PCR primers]

'''Team Progress Table'''

{| border="1" cellpadding="2"
!width="200"|Student Name
!width="30"|5mer Codon
!width="40"|Anticodon Sequence
!width="40"| [http://gcat.davidson.edu/GcatWiki/index.php/What_naming_system_are_we_going_to_use_for_the_suppressor_tRNAs%3F Codon Short Name]
!width="150"|tRNA Status
!width="150"|5mer+RFP Status
!width="150"|5mer+Tet Resistance Status
|- 
| Olivia Ho-Shing || CCAUC || GUGAUCCAA-9 || Pro4 || 4 clones being sequenced || Amplifying 5mer+RFP || Amplifying 5mer+Tet
|-   
| Olivia Ho-Shing || CCAUC || UUUGAUGGAG-10 || Pro5 || 3 clones being sequenced || Amplifying 5mer+RFP || Amplifying 5mer+Tet
|-   
| Romina Clemente|| CUAGU || UUACUAGAC-9 || Leu4|| 1 clone being sequenced || Gel purifying 5mer+RFP || Gel purifying 5mer+Tet Resistance
|-   
| Shamita Punjabi|| CCACU || CUAGUGGAC-9 || Pro3|| 3 clones being sequenced || Cleaning/Concentrating 5mer+ RFP || Cleaning+Conc 5mer +Tet 
|-   
| Leland Taylor || CCCUC || CUGAGGGUC || Pro6 || 2 clones being sequenced || Transformed 5mer+RFP || Transformed 5mer+Tet
|-   
| Alyndria Thompson|| CGGUC || UUGACCGAC || Arg2 || 5 clones being sequenced || Transformed || Transformed
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
| Student Name|| 5mer codon || Anticodon Sequence || Codon Short Name|| tRNA Status || 5mer+RFP Status || 5mer+Tet Resistance Status
|-   
|}


Math Based: (Davidson) We have posted a Mathematica notebook on the Wiggio. Basically we are at a point where we can choose the number of variables, SAT number(2-SAT, 3-SAT, etc), and the number of clauses to use at a time and produce a table of inputs as the rows and clauses as the columns, with a 1 if the input satisfies the clause and a 0 if the input fails to satisfy the clause. In addition, we can produce a "Super Table," which lists all the SAT problems given the number of clauses in conjunctive normal form and displays how many of the clauses in a given problem each input satisfies. Feel free to view the posted Mathematica notebook; there are some instructions within the notebook itself. When you open it, there will be a window which asks about dynamic content; be sure to click "Enable Dynamic."