View source for Prepare PCR product for sequencing

You have amplified your TAS2R38 alleles for sequencing. Now you need to mix the sequencing primer with your PCR product. 

# Pipet 8 µL of PCR product into the bar coded tube.
# Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
# Place the small cap back onto the bar coded sequencing tube. 
# Record the bar code number of your tube. Write your name next to the bar code number on the sheet in lab.
# The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon].