CRISPR-associated Proteins - Revision history

Sapyfrom at 16:55, 10 November 2009

2009-11-10T16:55:57Z

Sapyfrom at 16:55, 10 November 2009

2009-11-10T16:55:25Z

Sapyfrom at 16:54, 10 November 2009

2009-11-10T16:54:45Z

Sapyfrom at 16:46, 10 November 2009

2009-11-10T16:46:41Z

Sapyfrom at 17:44, 5 November 2009

2009-11-05T17:44:05Z

Sapyfrom at 17:43, 5 November 2009

2009-11-05T17:43:40Z

Sapyfrom at 17:37, 5 November 2009

2009-11-05T17:37:09Z

Sapyfrom at 17:05, 5 November 2009

2009-11-05T17:05:48Z

Sapyfrom at 17:04, 5 November 2009

2009-11-05T17:04:33Z

Sapyfrom at 17:04, 5 November 2009

2009-11-05T17:04:23Z

← Older revision		Revision as of 16:55, 10 November 2009
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	6) TM1801 family		6) TM1801 family

		+	[[Cas-Protein aa sequences]]

	'''After comparing the conserved proteins between our genome and the other 9 genomes, I found these cas-proteins were conserved:'''		'''After comparing the conserved proteins between our genome and the other 9 genomes, I found these cas-proteins were conserved:'''

@@ Line 42: / Line 42: @@
 Usually found usually in the order cas3-cas4-cas1-cas2.
-'''Huzzah!''' Our genomes Cas-proteins appear in the same order! (Well....2-1-4-3...but still!)
+'''Huzzah!''' Our genome's Cas-proteins appear in the same order! (Well....2-1-4-3...but still!)
 "CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04 SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA]

← Older revision		Revision as of 16:54, 10 November 2009
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	Usually found usually in the order cas3-cas4-cas1-cas2.		Usually found usually in the order cas3-cas4-cas1-cas2.
		+
		+	'''Huzzah!''' Our genomes Cas-proteins appear in the same order! (Well....2-1-4-3...but still!)

	"CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04 SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA]		"CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04 SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA]

@@ Line 34: / Line 34: @@
 H. utahensis shares ''all 6 cas-proteins'' with H. mukohataei.
 '''Research:'''
 Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.
 "CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04 SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA]

← Older revision		Revision as of 17:44, 5 November 2009
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	Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.		Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.

−	"CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04]	+	"CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04 SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA]

← Older revision		Revision as of 17:43, 5 November 2009
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	Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.		Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.
		+
		+	"CRISPR sequences have been found to be flanked by protein genes named cas (CRISPR-associated) genes and their encoded proteins are therefore called CAS proteins [2]. They are typically organized in small clusters and are associated with specific subtypes of CRISPRs. The number of cas genes associated with CRISPR subtypes is also variable, ranging from 6 to more than 20. The specific domains of CAS proteins have been predicted to harbor nuclease, helicase, recombinase, integrase and RNA and/or DNA binding activities [6] and [7]."[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4W7RYGM-3&_user=2665120&_coverDate=06%2F18%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4938&_sort=d&_docanchor=&view=c&_searchStrId=1079748718&_rerunOrigin=google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=6969fb4632d0d7e6c5856f37a426cc04]

← Older revision		Revision as of 17:37, 5 November 2009
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	H. utahensis shares ''all 6 cas-proteins'' with H. mukohataei.		H. utahensis shares ''all 6 cas-proteins'' with H. mukohataei.
		+
		+	'''Research:'''
		+
		+	Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.

@@ Line 17: / Line 17: @@
 '''After comparing the conserved proteins between our genome and the other 9 genomes, I found these cas-proteins were conserved:'''
-H. sinaiiensis shares all 6 cas-proteins with H. mukohataei.
+H. sinaiiensis shares ''all 6 cas-proteins'' with H. mukohataei.
-H. vallismortis shares all cas-proteins except for Cas4.
+H. vallismortis shares ''all cas-proteins except for Cas4.''
-H. californiae shares all cas-proteins except for Cas4.
+H. californiae shares ''all cas-proteins except for Cas4.''
-H. denitrificans shares all 6 cas-proteins with H. mukohataei.
+H. denitrificans shares ''all 6 cas-proteins'' with H. mukohataei.
-H. mediteranei shares all 6 cas-proteins with H. mukohataei.
+H. mediteranei shares ''all 6 cas-proteins'' with H. mukohataei.
-H. volcanii shares only Cas2, Cas3 and Cas4 with H. mukohataei.
+H. volcanii shares ''only Cas2, Cas3 and Cas4'' with H. mukohataei.
-H. sulfurifontis shares only Cas1, Cas3 and TM1800.
+H. sulfurifontis shares ''only Cas1, Cas3 and TM1800''.
-H. mucosum shares all 6 cas-proteins with H. mukohataei.
+H. mucosum shares ''all 6 cas-proteins'' with H. mukohataei.
-H. utahensis shares all 6 cas-proteins with H. mukohataei.
+H. utahensis shares ''all 6 cas-proteins'' with H. mukohataei.

@@ Line 1: / Line 1: @@
 '''RAST and JGI agree that our genome has 6 CRISPR-associated (cas) proteins:
 '''
 ) Cas1