Cellulase - Revision history

PaPenumetcha at 15:56, 13 November 2008

2008-11-13T15:56:07Z

2008-11-13T15:53:44Z

2008-11-13T15:51:24Z

2008-11-04T16:53:31Z

2008-11-04T16:51:33Z

2008-11-04T16:50:45Z

2008-11-04T16:17:43Z

2008-11-04T16:17:21Z

2008-11-04T16:16:47Z

2008-11-04T16:15:49Z

@@ Line 8: / Line 8: @@
 Enzyme 3.2.1.19 is the cellobiohydrolase <br>
 Enzyme 3.2.1.12 is the beta-glucosidase <br>
-I think it would be interesting to directly test for the enzymes highlighted in red and allow the organism to grow in the absence of
+I think it would be interesting to directly test for the enzymes highlighted in red and allow the organism to grow in the absence of glucose. I think that performing these two experiments will give us a lot of insight into how this organism produces glucose, because if we see that the organism can grow in the absence of the glucose and that it doesn't have some of these enzymes highlighted in red, this would suggest that this organism might have a different pathway for breaking down cellulose.

@@ Line 8: / Line 8: @@
 Enzyme 3.2.1.19 is the cellobiohydrolase <br>
 Enzyme 3.2.1.12 is the beta-glucosidase <br>
-I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.
+I think it would be interesting to directly test for the enzymes highlighted in red and allow the organism to grow in the absence of

@@ Line 8: / Line 8: @@
 Enzyme 3.2.1.19 is the cellobiohydrolase <br>
 Enzyme 3.2.1.12 is the beta-glucosidase <br>
-[[Image:ECBLASTer.png]] <br>
+I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.

@@ Line 8: / Line 8: @@
 Enzyme 3.2.1.19 is the cellobiohydrolase <br>
 Enzyme 3.2.1.12 is the beta-glucosidase <br>
 [[Image:ECBLASTer.png]] <br>
 According to the EC BLASTer, our organism does not contain enzymes 2.7.7.34 or 2.4.1.29. I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.

@@ Line 8: / Line 8: @@
 Enzyme 3.2.1.19 is the cellobiohydrolase <br>
 Enzyme 3.2.1.12 is the beta-glucosidase <br>
 [[Image:ECBLASTer.png]] <br>
 According to the EC BLASTer, our organism does not contain enzymes 2.7.7.34 or 2.4.1.29. I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.

@@ Line 6: / Line 6: @@
 Purple: Molecule or Protein <br>
 Enzyme 3.2.1.4 is the cellulase <br>
 [[Image:ECBLASTer.png]] <br>
 According to the EC BLASTer, our organism does not contain enzymes 2.7.7.34 or 2.4.1.29. I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.

← Older revision		Revision as of 16:16, 4 November 2008
Line 6:		Line 6:
	Purple: Molecule or Protein <br>		Purple: Molecule or Protein <br>
	[[Image:ECBLASTer.png]] <br>		[[Image:ECBLASTer.png]] <br>
		+	According to the EC BLASTer, our organism does not contain enzymes 2.7.7.34 or 2.4.1.29. I think it would be interesting to either directly test for these two enzymes, or give the organism Alpha-D-Glucose 1P and see if it can produce Beta-D-Glucose. If the organism doesn’t have these two enzymes, that would also be very interesting, because it would suggest that it has a different way of forming glucose. I also think it would be interesting to directly test for enzymes 2.7.7.9 and 2.4.1.12, because all three databases did not call these enzymes, but it looks like there could be sequences associated with these enzymes in our organism’s genome.

@@ Line 1: / Line 1: @@
-[[Image:Cellulase.png]]
+[[Image:Cellulase.png]] <br>
-Legend:
+Legend:<br>
-Green: ''H. utahensis'' has the enzyme
+Green: ''H. utahensis'' has the enzyme <br>
 Red: "H. utahensis" does not have the enzyme <br>
 Blue: Pathway <br>
 Purple: Molecule or Protein <br>
 [[Image:ECBLASTer.png]] <br>