Chitinase - Revision history

Malotz at 15:13, 4 December 2008

2008-12-04T15:13:52Z

Malotz at 15:11, 4 December 2008

2008-12-04T15:11:44Z

Malotz at 16:10, 18 November 2008

2008-11-18T16:10:06Z

Malotz at 15:58, 18 November 2008

2008-11-18T15:58:59Z

Malotz at 15:56, 18 November 2008

2008-11-18T15:56:27Z

Malotz at 15:52, 18 November 2008

2008-11-18T15:52:04Z

Malotz at 15:50, 18 November 2008

2008-11-18T15:50:12Z

Malotz at 15:49, 18 November 2008

2008-11-18T15:49:06Z

Malotz at 16:11, 6 November 2008

2008-11-06T16:11:37Z

Malotz at 16:10, 6 November 2008

2008-11-06T16:10:39Z

@@ Line 5: / Line 5: @@
 The only automated annotation database to predict a chitinase was Manatee. Manatee predicted Chitinase A at 55855...57810. [http://www.tigr.org/tigr-scripts/prok_manatee/shared/ORF_infopage.cgi?db=nthu01&orf=ORF00052 Chitinase A: Manatee]<br>
 The top BlastP results:  [[Image:blastpr.png]]<br><br>
-As you can see, Chitinase A is not among the top hits. However, there is one hit for it further down the list:<br>
+As you can see, Chitinase A is not among the top hits. However, there is one hit for it further down the list with a good e-value:<br>
 [[Image:blastpr2.png]]<br>
 So, this location possibly codes for chitinase.<br>
@@ Line 13: / Line 13: @@
 Second Chitinase hit: [[Image:ChitinaseBlast2.png]] <br> <br>
-So, data from this coding region strongly suggest the coding of chitinase in our organism. However, we do not yet have any physiological data to support the presence of chitinase.
+Both of these hits had extremely good e-values; so, data from this coding region strongly suggest the coding of chitinase in our organism. However, we do not yet have any physiological data to support the presence of chitinase.

@@ Line 7: / Line 7: @@
 As you can see, Chitinase A is not among the top hits. However, there is one hit for it further down the list:<br>
 [[Image:blastpr2.png]]<br>
-So, although it is possible that Chitinase is coded for by this region, there is a stronger possibility that it is not.<br>
+So, this location possibly codes for chitinase.<br>
 <br><br>
 While doing some hand-curated annotations, we also found another potential chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp that coded for chitinase, they were the 2nd and 3rd hits overall and both were from halophiles: <br>

@@ Line 1: / Line 1: @@
 KEGG Pathway containing Chitinase: Aminosugars Metabolism
 [[Image:pathway.png]]<br>
-The pathway above is labeled for a halophile, haloarcula marismortui (most of the other halophiles on KEGG did not have a reference pathway for Aminosugars Metabolism). As you can see, Chitinase (EC#:3.2.1.14) is not predicted for this halophile.<br>
+The pathway above is labeled for a halophile, haloarcula marismortui (most of the other halophiles on KEGG did not have a reference pathway for Aminosugars Metabolism). As you can see, chitinase (EC#:3.2.1.14) is not predicted for this halophile.<br>
 <br><br>
-The only automated annotation database to predict a Chitinase was Manatee. Manatee predicted Chitinase A at 55855...57810. [http://www.tigr.org/tigr-scripts/prok_manatee/shared/ORF_infopage.cgi?db=nthu01&orf=ORF00052 Chitinase A: Manatee]<br>
+The only automated annotation database to predict a chitinase was Manatee. Manatee predicted Chitinase A at 55855...57810. [http://www.tigr.org/tigr-scripts/prok_manatee/shared/ORF_infopage.cgi?db=nthu01&orf=ORF00052 Chitinase A: Manatee]<br>
 The top BlastP results:  [[Image:blastpr.png]]<br><br>
 As you can see, Chitinase A is not among the top hits. However, there is one hit for it further down the list:<br>
@@ Line 9: / Line 9: @@
 So, although it is possible that Chitinase is coded for by this region, there is a stronger possibility that it is not.<br>
 <br><br>
-While doing some hand-curated annotations, we also found another potential Chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp that coded for Chitinase, they were the 2nd and 3rd hits overall and both were from halophiles: <br>
+While doing some hand-curated annotations, we also found another potential chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp that coded for chitinase, they were the 2nd and 3rd hits overall and both were from halophiles: <br>
 First Chitinase hit: [[Image:ChitinaseBlast.png]] <br> <br>
-Second Chitinase hit: [[Image:ChitinaseBlast2.png]] <br>
+Second Chitinase hit: [[Image:ChitinaseBlast2.png]] <br> <br>

@@ Line 10: / Line 10: @@
 <br><br>
 While doing some hand-curated annotations, we also found another potential Chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp that coded for Chitinase, they were the 2nd and 3rd hits overall and both were from halophiles: <br>
-First Chitinase hit: [[Image:ChitinaseBlast.png]] <br>
+First Chitinase hit: [[Image:ChitinaseBlast.png]] <br> <br>
 Second Chitinase hit: [[Image:ChitinaseBlast2.png]] <br>

@@ Line 9: / Line 9: @@
 So, although it is possible that Chitinase is coded for by this region, there is a stronger possibility that it is not.<br>
 <br><br>
-While doing some hand-curated annotations, we also found another potential Chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp: <br>
+While doing some hand-curated annotations, we also found another potential Chitinase-coding region at 72986...74821 (+). Two hits were found by Blastp that coded for Chitinase, they were the 2nd and 3rd hits overall and both were from halophiles: <br>
-First Chitinase hit: [[Image:ChitinaseBlast.png]]
+First Chitinase hit: [[Image:ChitinaseBlast.png]] <br>

← Older revision		Revision as of 16:11, 6 November 2008
Line 9:		Line 9:
	So, although it is possible that Chitinase is coded for by this region, there is a stronger possibility that it is not.<br>		So, although it is possible that Chitinase is coded for by this region, there is a stronger possibility that it is not.<br>
	<br><br>		<br><br>
		+	While doing some hand-curated annotations, we also found another potential Chitinase-coding region.