Describe cellular communication systems used - Revision history

BoCool at 14:53, 28 October 2008

2008-10-28T14:53:01Z

BoCool at 14:51, 28 October 2008

2008-10-28T14:51:40Z

BoCool at 14:50, 28 October 2008

2008-10-28T14:50:38Z

BoCool at 14:50, 28 October 2008

2008-10-28T14:50:06Z

New page

== Las/Rhl cell signaling system ==
'''Responsible''': Robert Cool, Alicia Allen, and Erin Feeney

'''Las System'''

'''Signal Molecule''': An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)

'''Bacterial species''': Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)

'''Receiver protein''': LasR

'''Effect of binding''': TXN activation of virulence genes, lasA, lasB, apr, toxR

'''Synthase''': LasI enzyme

'''Target Genes''': lasI, lasA, lasB, apr, toxR

'''Regulation''': unknown

'''References'''

"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"

JP Pearson, EC Pesci and BH Iglewski
[http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=las&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]

"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"

Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci
[http://jb.asm.org/cgi/content/full/187/13/4372?view=long&pmid=15968046 2]

Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden
[http://jb.asm.org/cgi/content/full/185/12/3558?view=long&pmid=12775693 3]

Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]

'''Rhl System'''

<br>
'''Signal Molecule''': An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)

'''Bacterial species''': Pseudomonas aeruginosa, gram(-)

'''Receiver Protein''': Rhl R

'''Effect of Binding''': activation of Rhamnosyl Transferase, then making RL (rhamnolipid)

'''Synthase''': RhlA and RhlB

'''Target Genes''': pqsABCDE and phnAB

'''Regulation''': unknown

'''References'''

[http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]

[[Image:Las_rhl.gif]]

'''Parts Needed''':

LasR + pro/term

RhlR + pro/term

RhlI + pro/term

pqsABCDE + pro/term

pqsR

pqsH + pro/term

phnAB

LasI

RBS

@@ Line 63: / Line 63: @@
 [[Image:Las_rhl.gif]]
 == Lux cell signaling system ==
@@ Line 113: / Line 94: @@
 [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation
 == Lsr (AI-2) cell signaling system ==
@@ Line 149: / Line 121: @@
 '''Note:''' AI-2 is synthesized and secreted during exponential growth and is imported in stationary phase when glucose becomes limiting. In the presence of glucose, AI-2 is not imported because the lsr operon is not transcribed due to camp-CAP mediated repression. Both glycerol and G3P(glycerol 3-phosphate) repress lsr transcription, while the majority repression comes from G3P. DHAP represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR.
 http://gcat.davidson.edu/GcatWiki/images/b/b8/N654260305_1291548_2335.jpg

← Older revision		Revision as of 14:51, 28 October 2008
Line 122:		Line 122:

	LuxI sender		LuxI sender
		+
		+	== Lsr (AI-2) cell signaling system ==
		+
		+	'''Responsible''': Kelly Davis, Xiao Zhu
		+
		+	'''Signal molecule''': AI-2 (furanosyl borate diester in V. harveyi, a variety of other molecules in other species), all are derived from DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli]
		+
		+	[http://www.biomedcentral.com/content/pdf/1471-2148-4-36.pdf]
		+
		+	'''Bacterial species''':
		+
		+	lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.
		+
		+	lsrE:Escherichia coli str. K12 substr. MG1655
		+
		+	LuxS:Escherichia coli HS, SMS-3-5, APEC O1, str. K12 substr. MG1655, substr. DH10B, and UTI89.
		+
		+	'''Receiver protein''': LsrR protein receives signal from sensor protein
		+
		+	'''Signal molecule synthase''': Pfs enzyme, then LuxS autoinducer synthase
		+
		+	'''Target genes''': lsr operon, including ABC transporter and LsrK kinase
		+
		+	'''Regulation''': LsrR represses the lsr operon, derepression by phospho-AI-2; catabolite repression influences AI-2 accumulation through the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex, which directly stimulates transcription of the lsr operon and indirectly represses luxS expression.cAMP-CRP is shown to bind to a cAMP receptor protein (CRP) binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake.
		+
		+	'''Note:''' AI-2 is synthesized and secreted during exponential growth and is imported in stationary phase when glucose becomes limiting. In the presence of glucose, AI-2 is not imported because the lsr operon is not transcribed due to camp-CAP mediated repression. Both glycerol and G3P(glycerol 3-phosphate) repress lsr transcription, while the majority repression comes from G3P. DHAP represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR.
		+
		+	'''Parts Needed:'''
		+
		+	LsrR pro/term
		+
		+	LsrK
		+
		+	LsrACDB (transport)
		+
		+	LsrFGE (catabolic)
		+
		+	LuxS
		+
		+	Pfs enzyme (?)
		+
		+	http://gcat.davidson.edu/GcatWiki/images/b/b8/N654260305_1291548_2335.jpg
		+
		+	'''Note:'''
		+
		+	lsrB encodes the periplasmic AI-2 binding protein
		+
		+	lsrC & lsrD encode the channel proteins
		+
		+	lsrA encodes the ATPase that provides energy for AI-2 transport
		+
		+	lsrF is similar to genes specifying aldolases
		+
		+	lsrG encodes a protein with an unknown function.
		+
		+	[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5591389&ordinalpos=26&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum tam: trans-aconitate 2-methyltransferase, also known as lsrE or yneD] [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6061192&ordinalpos=10&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum yneE:conserved inner membrane protein]
		+
		+	http://gcat.davidson.edu/GcatWiki/images/3/30/N654260305_1340817_550.jpg
		+
		+	[[Image:Har.JPG]]
		+	[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=55669965 R-THMF]
		+	http://gcat.davidson.edu/GcatWiki/images/8/8c/S-DPD.gif
		+	http://www.nature.com/nrmicro/journal/v3/n5/images/nrmicro1146-f2.gif
		+
		+	http://gcat.davidson.edu/GcatWiki/images/c/c9/Grl.jpg
		+
		+	DHAP: dihydroxyacetone phosphate.
		+
		+	http://gcat.davidson.edu/GcatWiki/images/9/9b/Grlw.jpg
		+
		+	'''References'''
		+
		+	[http://jb.asm.org/cgi/content/full/187/6/2066?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=lsrR&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Cyclic AMP and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli]
		+
		+	[http://iai.asm.org/cgi/reprint/IAI.00550-07v1.pdf Global Effects of the Cell-to-Cell Signaling Molecules Autoinducer-2, Autoinducer-3, and Epinephrine in a luxS Mutant of Enterohemorrhagic Escherichia Coli]
		+
		+	[http://www.rsc.org/delivery/_ArticleLinking/DisplayHTMLArticleforfree.cfm?JournalCode=CC&Year=2005&ManuscriptID=b509396a&Iss=38 Shows how AI-2 is formed]
		+
		+	[http://www.jstor.org/sici?sici=0027-8424(20031125)100%3C14549%3ACCAB%3E2.0.CO%3B2-B&cookieSet=1 Signaling explained with graphics of AI-2 pathways]
		+
		+	[http://web.ebscohost.com/ehost/detail?vid=1&hid=116&sid=edfbf2f7-b0c8-40c3-8227-1cc94f134972%40sessionmgr108 Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium]
		+
		+	[http://www.microbialcellfactories.com/content/pdf/1475-2859-1-5.pdf Review of AI-2 and other systems]
		+
		+	[http://jb.asm.org/cgi/content/full/189/16/6011?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=AI+2+LsrR&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture]
		+
		+	[http://jb.asm.org/cgi/content/full/187/1/238?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=quorum+sensing+AI-2&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli]
		+
		+	'''Resources'''
		+
		+	[http://www.ommscientific.com/ AI-2/DPD]
		+
		+	[http://www.biomedcentral.com/content/pdf/1471-2180-8-98.pdf alternate AI-2 synthesis]
		+
		+	[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=5586283 lsrK gene in Entrez]
		+
		+	[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6062136&ordinalpos=9&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum#summary lsrR gene in Entrez]
		+
		+	[http://BioCyc.org/ECOLI/substring-search?type=NIL&object=lsr lsr nucleotide sequence in EcoCyc]
		+
		+	Transcription of LuxS (interaction with micA,gshA)
		+
		+	http://gcat.davidson.edu/GcatWiki/images/c/cb/N654260305_1347432_6212.jpg

← Older revision		Revision as of 14:50, 28 October 2008
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	RBS		RBS
		+
		+	== Lux cell signaling system ==
		+
		+	'''Responsible:''' Andrew Gordon and Pallavi Penumetcha
		+
		+	'''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules
		+
		+	'''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli''
		+
		+	'''Receiver protein:''' LuxR protein receives signal from AHL; also has some control over transciption of luciferase
		+
		+	'''Signal molecule synthase:''' LuxI; also has some control over transciption of luciferase
		+
		+	'''Additional Information:''' "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
		+
		+	[[Image:800px-Luxrreceiverschematic.png]]
		+
		+	'''Resources'''
		+
		+	[http://partsregistry.org/Lux Lux Operon Pathway]
		+
		+	[http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]
		+
		+	[http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]
		+
		+	'''References'''
		+
		+	[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]
		+
		+	[http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation
		+
		+	'''Parts Needed''':
		+
		+	LuxR + pro/term
		+
		+	RBS
		+
		+	LuxI + pro/term
		+
		+	LuxI sender