BoCool at 15:18, 28 October 2008

2008-10-28T15:18:45Z

New page

== Davidson XOR Biological Design ==
[http://partsregistry.org/AHL '''List of auto-inducers and their catalog numbers.''']
 
<center>'''Davidson Approach''' 
'''Here is an idea Malcolm and Laurie developed.''' 
'''Everyone please look at this and ask questions and find holes in it now so we don't waste time building something that won't work.''' </center>

<center> [[Image:XOR_AMC1b.jpg]] </center>
The idea is to have two mirrored halves of the system. LasR is regulated by PAI-1 {3-oxododecanoyl-HSL (3OC12HSL)} and LuxR is activated by AI-1 {3-oxohexanoyl-homoserine lactone ([http://partsregistry.org/3OC6HSL 3OC6HSL])}. There is a potential problem in that the Lux half is more likely to get positive feedback than the Las half. This MAY not be a problem because 0/0 is leaky so we put a weak RBS to minimize leaky protein production. Also, if we add AI-2 and AI-1 is produced by leak, then the entire system shuts down. The repressor site is located between -35 and -10 of the promoter. The activator binding site is upstream of -35. This has been documented [http://www.bio.davidson.edu/courses/synthetic/papers/LuxR.pdf by Egland and Greenberg]

[[Oligos_to_Build]]: Sequences we will need to make this XOR gate.

== Missouri Western XOR Biological Design 1==

These two XOR circuits are designed to complement each other. Each recieves a cell-to-cell signal (AI-1 or AI-2) and a chemical signal (IPTG or AHL) and processes it into a cell-to-cell signal. Colonies that output AI-1 would alternate with colonies that produce AI-2. The input message to be hashed could be encoded by the presence or absence of the chemical signals, which would also alternate.

[[Image:XOR DR AI2.PNG]]

'''Above '''- Input of AI-1 or IPTG turns on production of AI-2 by LuxS. Input by both AI-1 and IPTG allows production of the repressors cI and Mnt, which repress both transcription units. LuxR and LacI are constitutively expressed.

[[Image:XOR DR AI1b.PNG]] 
'''Above''' - Input of AI-2 or aTc turns on production of AI-1 by LuxI. Input by both AI-2 and aTc allows production of the repressors cI and Mnt, which repress both transcription units. LsrK, LsrR and TetR are constitutively expressed.

Describe different design architectures - Revision history

BoCool at 15:18, 28 October 2008