http://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=Direct_Synthesis_with_Overlapping_OligosDirect Synthesis with Overlapping Oligos - Revision history2026-05-17T07:56:11ZRevision history for this page on the wikiMediaWiki 1.28.2http://gcat.davidson.edu/GcatWiki/index.php?title=Direct_Synthesis_with_Overlapping_Oligos&diff=5218&oldid=prevToEckdahl at 20:36, 12 June 20082008-06-12T20:36:24Z<p></p>
<p><b>New page</b></p><div> Design oligos with biobrick suffix, prefix, and at least 15 bp overlap.<br />
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Assemble reaction for PCR.<br />
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1 ul (10 ug) of oligo A<br />
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1 ul (10)ug) of oligo B<br />
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4 ul 10X PCR buffer w/ 15 mM MgCl2<br />
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2 ul 10X dNTDs <br />
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1 ul Taq<br />
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31 ul dH2O<br />
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5 cycles: 94 C 15 sec.; annealing temp. 15 sec.; 74 C 30 sec.<br />
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annealing temp. must be 4 C lower than Tm of either oligo.</div>ToEckdahl