Gel Electrophoresis for Bio113 - Revision history

Macampbell at 15:49, 19 July 2017

2017-07-19T15:49:54Z

Macampbell at 15:49, 19 July 2017

2017-07-19T15:49:20Z

Macampbell at 20:47, 18 July 2017

2017-07-18T20:47:24Z

Macampbell: Created page with "Analyzing PCR Results Using Gel Electrophoresis # A 2.0% agarose gel has been poured for you. It contains 0.5X TBE buffer. # Your will start with the tubes containing your..."

2017-07-18T20:44:32Z

Created page with "Analyzing PCR Results Using Gel Electrophoresis # A 2.0% agarose gel has been poured for you. It contains 0.5X TBE buffer. # Your will start with the tubes containing your..."

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Analyzing PCR Results Using Gel Electrophoresis

# A 2.0% agarose gel has been poured for you. It contains 0.5X TBE buffer.
# Your will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube.
# Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load.
# Dr. C. will load 5 µL of the 1 kb molecular weight marker (also called 1 kb ladder).
# The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA.
# The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results.

@@ Line 4: / Line 4: @@
 # You will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube.
 # Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load.
-# Dr. C. will load 5 µL of the 1 kb molecular weight marker ([http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/1kbladder.png also called 1 kb ladder).
+# Dr. C. will load 5 µL of the 1 kb molecular weight marker ([http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/1kbladder.png also called 1 kb ladder]).
 # The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA.
 # The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results.