IGEM 2010 Project - Revision history

Jmpeterson at 19:41, 7 July 2010

2010-07-07T19:41:30Z

2010-07-01T15:40:15Z

‎Biology Sub-Projects

2010-07-01T13:22:56Z

‎Math Sub-Projects

2010-07-01T13:20:09Z

‎Math Sub-Projects

2010-06-30T20:13:33Z

‎Biology Sub-Projects

2010-06-28T20:47:26Z

‎Math Sub-Projects

2010-06-28T19:18:29Z

‎Math Sub-Projects

2010-06-28T19:11:52Z

‎Math Sub-Projects

2010-06-24T18:24:08Z

‎Biology Sub-Projects

2010-06-24T18:16:36Z

‎Biology Sub-Projects

@@ Line 325: / Line 325: @@
 |<span style="color:#B22222"> '''loxN Reverse in pSB1A2''' </span>  || <span style="color:#B22222"> I had 3 successful colonies from this oligos ligation that were also confirmed by colony PCR. They are sent off for sequencing and two of them were confirmed to be correct.  ||  [http://partsregistry.org/Part:BBa_K315011] </span> <br> <span style="color:#B22222"> loxN reverse does not exist in the registry. We ordered the oligos and built it in the lab.  </span> || Anvi
 |-
 |}

← Older revision		Revision as of 13:22, 1 July 2010
Line 123:		Line 123:

	\|}		\|}
		+
		+	This is a chart of the experimental group in the Paris IGEM 2007 project. I used the formula G=log(1/200)/log(1-r) where r is the recombination rate (how many times Cre makes a cut and a Scar site is formed) and G is the number of generations (or the number of times the cell will need to divide). I am making an assumption here that the initial prey population (the plasmids in the cell) is 200.

	== Biology Sub-Projects==		== Biology Sub-Projects==

← Older revision		Revision as of 13:20, 1 July 2010
Line 58:		Line 58:

	This is a chart of the experimental group in the Paris IGEM 2007 project. I used the formula r=1-10^(.004*T)/2 where r is the recombination rate (how many times Cre makes a cut and a Scar site is formed) and T is the generation time in minutes (How long it takes the cell to divide).		This is a chart of the experimental group in the Paris IGEM 2007 project. I used the formula r=1-10^(.004*T)/2 where r is the recombination rate (how many times Cre makes a cut and a Scar site is formed) and T is the generation time in minutes (How long it takes the cell to divide).
		+
		+	'''Paris IGEM 2007 Results'''
		+	{\| class="wikitable" style="text-align:center" border="1" cellpadding="2"
		+	\|'''r'''
		+	\|'''G'''
		+	\|-
		+
		+	\|.05 \|\|103.3
		+	\|-
		+
		+	\|.10 \|\|50.3
		+	\|-
		+
		+	\|.15 \|\|32.6
		+	\|-
		+
		+	\|.20 \|\|23.7
		+	\|-
		+
		+	\|.25 \|\|18.4
		+	\|-
		+
		+	\|.30 \|\|14.9
		+	\|-
		+
		+	\|.35 \|\|12.3
		+	\|-
		+
		+	\|.40 \|\|10.4
		+	\|-
		+
		+	\|.45 \|\|8.9
		+	\|-
		+
		+	\|.50 \|\|7.6
		+	\|-
		+
		+	\|.55 \|\|6.6
		+	\|-
		+
		+	\|.60 \|\|5.8
		+	\|-
		+
		+	\|.65 \|\|5.0
		+	\|-
		+
		+	\|.70 \|\|4.4
		+	\|-
		+
		+	\|.75 \|\|3.8
		+	\|-
		+
		+	\|.80 \|\|3.3
		+	\|-
		+
		+	\|.85 \|\|2.8
		+	\|-
		+
		+	\|.90 \|\|2.3
		+	\|-
		+
		+	\|.95 \|\|1.8
		+	\|-
		+
		+	\|}

	== Biology Sub-Projects==		== Biology Sub-Projects==

@@ Line 21: / Line 21: @@
 http://gcat.davidson.edu/iGem10/index.html
-'''Paris IGEM 2007 Results'''
+|20.5 ||39.61
+|-
-.5         40.16
+|25.5 ||36.76
-.5	     39.61
+|30.5 ||33.78
-.5	     36.76
+|35.5 ||30.66
-.5	     33.78
+|40.5 ||27.39
-.5	     30.66
+|45.5 ||23.97
-.5	     27.39
+|50.5 ||20.39
-.5	     23.97
+|55.5 ||16.64
-.5	     20.39
+|}
 This is a chart of the experimental group in the Paris IGEM 2007 project. I used the formula r=1-10^(.004*T)/2 where r is the recombination rate (how many times Cre makes a cut and a Scar site is formed) and T is the generation time in minutes (How long it takes the cell to divide).

← Older revision		Revision as of 19:11, 28 June 2010
Line 20:		Line 20:

	http://gcat.davidson.edu/iGem10/index.html		http://gcat.davidson.edu/iGem10/index.html
		+
		+
		+
		+	'''Paris IGEM 2007 Results'''
		+	T r
		+	19.5 40.16
		+	20.5 39.61
		+	25.5 36.76
		+	30.5 33.78
		+	35.5 30.66
		+	40.5 27.39
		+	45.5 23.97
		+	50.5 20.39
		+	55.5 16.64
		+
		+	This is a chart of the experimental group in the Paris IGEM 2007 project. I used the formula r=1-10^(.004*T)/2 where r is the recombination rate (how many times Cre makes a cut and a Scar site is formed) and T is the generation time in minutes (How long it takes the cell to divide).

	== Biology Sub-Projects==		== Biology Sub-Projects==

@@ Line 239: / Line 239: @@
 |-
-|<span style="color:#B22222"> '''Insert Info Here''' </span>  || <span style="color:#B22222"> what is built so far?  ||  [http://partsregistry.org/Part:BBa_K199000 change to correct link] </span> <br> <span style="color:#B22222"> describe intermediate </span> || your name here
+|<span style="color:#B22222"> '''I718008 (pBAD+RBS+cre) in pSB3K3''' </span>  || <span style="color:#B22222"> At this point, I have ligated and transformed the cells but I am having trouble growing it in the LB + kan media. ||  [http://partsregistry.org/Part:BBa_I718008 I718008][http://partsregistry.org/wiki/index.php?title=Part:pSB3K5] </span> <br> <span style="color:#B22222"> describe intermediate </span> || Anvi
 |-

@@ Line 216: / Line 216: @@
 |<span style="color:#B22222"> '''loxN Forward in pSB1A2''' </span>  || <span style="color:#B22222"> I had 5 successful colonies from this oligos ligation that were also confirmed by colony PCR. They are sent off for sequencing and four of them were confirmed to be correct.  ||  [http://partsregistry.org/Part:BBa_K315007] </span> <br> <span style="color:#B22222"> loxN forward does not exist in the registry. We ordered the oligos and built it in the lab.  </span> || Anvi
 |-
 |}
 <br>