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LuxR/cI Test and Outcome - Revision history
2026-07-01T16:33:29Z
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AnGordon at 19:44, 30 June 2008
2008-06-30T19:44:37Z
<p></p>
<p><b>New page</b></p><div>'''Test pLuxR/cI'''<br />
<br />
luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.<br />
<br />
Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glows<br />
<br />
<br />
add AI-1<br />
<br />
Some promoter→LuxR+t+t+pLuxR/cI→GFP glows<br />
<br />
add AI-1<br />
<br />
Some promoter→LuxR+cI+t+t+pLuxR/cI→GFP does not glow<br />
<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LuxR<br />
!width="30"|cI<br />
!width="30"|AI-1 + GFP<br />
!width="30"|AI-1 - GFP<br />
|-<br />
| 0 || 0 || 0 || 0 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}</div>
AnGordon