PH Regulation - Revision history

Alvalauriorton: /* NHEs: Antiporters most important to pH */

2011-02-03T05:01:31Z

‎NHEs: Antiporters most important to pH

2011-02-01T04:59:59Z

‎Antiporter controls intracellular pH

2011-02-01T04:22:28Z

‎How should we use this information in our studies?

2011-02-01T04:21:41Z

‎How should we use this information in our studies?

2011-02-01T04:21:19Z

2011-02-01T04:04:50Z

‎NHEs: Antiporters most important to pH

2011-02-01T04:04:25Z

‎NHEs: Antiporters most important to pH

2011-02-01T04:03:12Z

‎NHEs: Antiporters most important to pH

2011-02-01T03:18:24Z

2011-02-01T03:17:13Z

← Older revision		Revision as of 05:01, 3 February 2011
Line 18:		Line 18:

	Other ion exchangers noted to have a key role in pH are AE1 and NBC1.		Other ion exchangers noted to have a key role in pH are AE1 and NBC1.
		+
		+
		+	The NHE family of genes is referred to as NHX in plants. This family includes NHX1, NHX2, etc.

	== Actual pH of blueberries ==		== Actual pH of blueberries ==

@@ Line 1: / Line 1: @@
 == Antiporter controls intracellular pH ==
-The literature suggests that a antiporters, specifically a Na+/H+ antiporter, are responsible for controlling pH within cells. [http://www.ncbi.nlm.nih.gov/pubmed/6325586 1][http://bass.bio.uci.edu/~hudel/m160/presentations/3_antiporter.pdf 2]
+The literature suggests that antiporters, specifically a Na+/H+ antiporter, are responsible for controlling pH within cells. [http://www.ncbi.nlm.nih.gov/pubmed/6325586 1][http://bass.bio.uci.edu/~hudel/m160/presentations/3_antiporter.pdf 2]
 As noted in a 2002 article, "Proton fluxes at the plasma membrane are regulated by several families of ion exchangers, including the Na+/H+ exchangers(NHEs) and HCO3 transporters, such as the Na+/HCO-3- cotransporters (NBCs), Cl−/HCO−3 exchangers (AEs), and the Na+ driven Cl−/HCO−3- exchanger (NDAE). [http://www.annualreviews.org/doi/pdf/10.1146/annurev.pharmtox.42.092001.143801 3]

@@ Line 30: / Line 30: @@
 == How should we use this information in our studies? ==
- In studying the blueberry genome, we should look for these key pH-regulating proteins and note any differences.
+In studying the blueberry genome, we should look for these key pH-regulating proteins and note any differences.
 - Are the same regions highly conserved?

@@ Line 29: / Line 29: @@
 == How should we use this information in our studies? ==
   In studying the blueberry genome, we should look for these key pH-regulating proteins and note any differences.
 - Are the same regions highly conserved?
 - Are key binding regions different in blueberry?

@@ Line 18: / Line 18: @@
 Other ion exchangers noted to have a key role in pH are AE1 and NBC1.
 == How should we use this information in our studies? ==
-In studying the blueberry genome, we should look for these key pH-regulating proteins and note any differences.  Are the same regions highly conserved?  Are key binding regions different in blueberry?  Is the C-terminus of NHE1 in blueberries any different, such that it reacts differently to changes in pH?
+ In studying the blueberry genome, we should look for these key pH-regulating proteins and note any differences.
+- Are the same regions highly conserved?
-The main antiporter in bacteria has been identified as NhaA. [http://bass.bio.uci.edu/~hudel/m160/presentations/3_antiporter.pdf 2]  This protein "uses the electrochemical proton gradient maintained across the bacterial membrane and excretes Na+ in exchange for a 'downhill' flow of protons into the cell." [http://bass.bio.uci.edu/~hudel/m160/presentations/3_antiporter.pdf 2]  Though NhaA plays a role in changing the pH of a cell, it is actually regulated by pH.  It is down-regulated in an acidic pH.
+Possible wet lab activity: if the berry and the leaves have such different pH's, what if we tested for the amount of pH-regulation-related proteins in the berry vs. the leave, to figure out which proteins might be most responsible for the pH of the berry?

@@ Line 14: / Line 14: @@
 Below is a graphic of the C-terminus of NHE1 and the predicted binding sites:<br>
 [[Image:NHE1.jpeg]]
-[http://www.annualreviews.org/doi/pdf/10.1146/annurev.pharmtox.42.092001.143801 3 photo source]
+[http://www.annualreviews.org/doi/pdf/10.1146/annurev.pharmtox.42.092001.143801 photo source]

← Older revision		Revision as of 04:03, 1 February 2011
Line 11:		Line 11:

	NHE1 is regulated by a number of other intracellular complexes. It has been noted that its C-terminus is especially important in determining its sensitivity to changes in pH.		NHE1 is regulated by a number of other intracellular complexes. It has been noted that its C-terminus is especially important in determining its sensitivity to changes in pH.
		+
		+	Below is a graphic of the C-terminus of NHE1 and the predicted binding sites:
		+	[[Image:NHE1.jpeg]]

	Other ion exchangers noted to have a key role in pH are AE1 and NBC1.		Other ion exchangers noted to have a key role in pH are AE1 and NBC1.