RRNA operon - Revision history

Salt at 06:59, 2 September 2008

2008-09-02T06:59:13Z

Salt at 05:27, 2 September 2008

2008-09-02T05:27:04Z

Salt at 04:01, 2 September 2008

2008-09-02T04:01:09Z

Salt at 03:38, 2 September 2008

2008-09-02T03:38:33Z

Salt: /* rRNA Operon */

2008-09-02T01:00:36Z

‎rRNA Operon

Salt: /* rRNA Operon */

2008-09-02T00:57:11Z

‎rRNA Operon

Salt: /* rRNA Operon */

2008-09-02T00:56:44Z

‎rRNA Operon

Salt: /* rRNA Operon */

2008-09-02T00:52:53Z

‎rRNA Operon

Salt: /* rRNA Operon */

2008-09-02T00:51:55Z

‎rRNA Operon

Salt: /* rRNA Operon */

2008-09-02T00:47:38Z

‎rRNA Operon

← Older revision		Revision as of 06:59, 2 September 2008
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	Along with a promoter, a termination sequence, and an operator, the three seperate segments coding for the different subunits of the ribosome all form a single operon. The specific promoter and termination sequence is not known, but a [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=ScaffoldGraph&page=scaffoldDna&scaffold_oid=2500575882&start_coord=2396557&end_coord=2397346 790-base-pair intergenetic region] exists upstream of the 16S coding region; the promoter sequence most likely resides there.		Along with a promoter, a termination sequence, and an operator, the three seperate segments coding for the different subunits of the ribosome all form a single operon. The specific promoter and termination sequence is not known, but a [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=ScaffoldGraph&page=scaffoldDna&scaffold_oid=2500575882&start_coord=2396557&end_coord=2397346 790-base-pair intergenetic region] exists upstream of the 16S coding region; the promoter sequence most likely resides there.

		+	[[Image:Diagram_operon.jpg]]
		+	<p><small>Fig. 1. Graphical representation of the rRNA operon. Coding sequences for rRNA are grouped into three distinct clusters, with 16S representing the small ribosomal subunit and 23S and 5S forming the large subunit. The 16S cluster contains a Shine-Dalgarno sequence near its 3' end, to allow the small ribosomal unit to bond with mRNA. The operon begins with a promoter sequence and an operator sequence, currently unidentified, and transcription is halted by a terminator sequence, also currently unidentified.</small>

		+	<br />

	{\| border="1" cellpadding="2"		{\| border="1" cellpadding="2"

← Older revision		Revision as of 05:27, 2 September 2008
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	The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the [http://en.wikipedia.org/wiki/Shine-Dalgarno_sequence Shine-Dalgarno sequence]. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end.		The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the [http://en.wikipedia.org/wiki/Shine-Dalgarno_sequence Shine-Dalgarno sequence]. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end.
		+
		+	Along with a promoter, a termination sequence, and an operator, the three seperate segments coding for the different subunits of the ribosome all form a single operon. The specific promoter and termination sequence is not known, but a [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=ScaffoldGraph&page=scaffoldDna&scaffold_oid=2500575882&start_coord=2396557&end_coord=2397346 790-base-pair intergenetic region] exists upstream of the 16S coding region; the promoter sequence most likely resides there.

← Older revision		Revision as of 04:01, 2 September 2008
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	----		----

		+	The rRNA operon encodes for the ribosomal RNA, which comprises the ribosomes. Three distinct rRNA sequences exist in the operon, encoding for the two subunits of the ribosome; the 5S and 23S sequences code for the large subunit, while the 16S sequence encodes for the small subunit.

		+	The three sequences are coded in the following order, from 3' to 5': 16S, 23S, 5S.
		+
		+	The 3' end of the 16S RNA, or small subunit, binds to the 5' end of the messenger RNA during transcription, specifically to the [http://en.wikipedia.org/wiki/Shine-Dalgarno_sequence Shine-Dalgarno sequence]. This aligns the mRNA's start codon for transcription. The 16S rRNA itself contains an anti-Shine-Dalgarno sequence on its 3' end.

@@ Line 1: / Line 1: @@
 ----
-All three rRNA sequences are in close proximity to one another, seperated by a few hundred base pairs.
@@ Line 8: / Line 8: @@
+|
 |-
-! Gene Name !! Gene Coordinates !! Number of Base Pairs (Length) !! Score of Nearest Homolog !! E-value of Nearest Homolog !! Percent Identity to nearest Homolog !!
+! Gene Name !! Gene Coordinates !! Number of Base Pairs (Length) !! Closest Match on BLAST (Homolog) !! Score of Nearest Match !! E-value of Nearest Match !! Percent Identity to Nearest Match
 |-
 ! '''16S rRNA'''
-| 2397347..2398825 || 1479 bp || 1966 || 0.0e+00 || 92.12%
+| 2397347..2398825 || 1479 bp || [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=TaxonDetail&page=taxonDetail&taxon_oid=638154504 Halobacterium sp. NRC - 1] || 1966 || 0.0e+00 || 92.12%
 |-
 ! '''Gap Bases'''
-| ''2398825..2399190'' || 365 || N/A || N/A || N/A ||
+| ''2398825..2399190'' || 365 || N/A || N/A || N/A || N/A
 |-
 ! '''23S rRNA'''
-| 2399190..2402100 || 2911 bp || 1899 || 0.0e+00 || 90.06%
+| 2399190..2402100 || 2911 bp || [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=TaxonDetail&page=taxonDetail&taxon_oid=637000187 Natronomonas pharaonis DSM 2160] || 1899 || 0.0e+00 || 90.06%
 |-
 ! '''Gap Bases'''
-| ''2402100..2402216'' || 116 || N/A || N/A || N/A ||
+| ''2402100..2402216'' || 116 || N/A || N/A || N/A ||N/A
 |-
 ! '''5S rRNA'''
-| 2402216..2402338 || 123bp || 143 || 3.0e-36 || 90.00%
+| 2402216..2402338 || 123bp || [http://imgweb.jgi-psf.org/cgi-bin/img_edu_v260/main.cgi?section=TaxonDetail&page=taxonDetail&taxon_oid=638154504 Halobacterium sp. NRC - 1] || 143 || 3.0e-36 || 90.00%
 |}

← Older revision		Revision as of 01:00, 2 September 2008
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−	~~= rRNA Operon =~~
−
	----		----

@@ Line 16: / Line 16: @@
 |-
 ! '''Gap Bases'''
-| 2 || 4 || N/A || N/A || N/A ||
+| 2 || 365 || N/A || N/A || N/A ||
 |-
 ! '''23S rRNA'''
@@ Line 22: / Line 22: @@
 |-
 ! '''Gap Bases'''
-| 4 || 8 || N/A || N/A || N/A ||
+| 4 || 116 || N/A || N/A || N/A ||
 |-
 ! '''5S rRNA'''
 | 2402216..2402338 || 123bp || 143 || 3.0e-36 || 90.00%
 |}