Status Of Building Parts - Revision history

MaCampbell at 14:14, 18 June 2008

2008-06-18T14:14:35Z

PaPenumetcha at 20:12, 17 June 2008

2008-06-17T20:12:58Z

PaPenumetcha at 20:12, 17 June 2008

2008-06-17T20:12:07Z

PaPenumetcha at 20:11, 17 June 2008

2008-06-17T20:11:15Z

PaPenumetcha at 20:07, 17 June 2008

2008-06-17T20:07:17Z

PaPenumetcha at 20:05, 17 June 2008

2008-06-17T20:05:06Z

PaPenumetcha at 20:02, 17 June 2008

2008-06-17T20:02:11Z

PaPenumetcha at 19:50, 17 June 2008

2008-06-17T19:50:16Z

PaPenumetcha at 19:42, 17 June 2008

2008-06-17T19:42:33Z

PaPenumetcha at 19:37, 17 June 2008

2008-06-17T19:37:00Z

← Older revision		Revision as of 14:14, 18 June 2008
Line 1:		Line 1:
	MWSU + Davidson Status of Building Sender and Receiver		MWSU + Davidson Status of Building Sender and Receiver
		+
		+	[http://partsregistry.org/AHL '''List of auto-inducers and their Sigma catalog numbers'''.]

	plux-lac hybrid promoter:		plux-lac hybrid promoter:

@@ Line 46: / Line 46: @@
 |BBa_C0079|| lasR activator|| 797|| Verified|| Yes
 |-
-|BBa_S03156|| Ribosomal binding site followed by lasr coding region|| Verified|| Yes
+|BBa_S03156|| Ribosomal binding site followed by lasr coding region|| 840|| Verified|| Yes
 |-
 |BBa_S03154|| Ribosomal binding site followed by lasI coding region|| 726||

@@ Line 48: / Line 48: @@
 |BBa_S03156|| Ribosomal binding site followed by lasr coding region|| Verified|| Yes
 |-
-|S03154|| Ribosomal binding site followed by lasI coding region|| 726||
+|BBa_S03154|| Ribosomal binding site followed by lasI coding region|| 726||
 |-
-|T9002||Inputs C06HSL and outputs GFP|| 1986||
+|BBa_T9002||Inputs C06HSL and outputs GFP|| 1986||
 |}
 -We will be sending all of these parts to be sequenced <br>

@@ Line 45: / Line 45: @@
 |-
 |BBa_C0079|| lasR activator|| 797|| Verified|| Yes
 |}
 -We will be sending all of these parts to be sequenced <br>

@@ Line 44: / Line 44: @@
 |BBa_J04450|| RFP coding device switched on by IPTG|| 2110 bp|| Much smaller on gel
 |-
-BBa_C0079||
+|BBa_C0079|| lasR activator|| 797|| Verified|| Yes
 |}
 -We will be sending all of these parts to be sequenced <br>

@@ Line 30: / Line 30: @@
 |BBa_C0261|| Produces luxI (w/ RBS)|| 702 bp|| No successful Mini-Preps
 |-
-|BBa_R0062|| luxR and HSL regulated promoter|| 96 bp|| Verified
+|BBa_R0062|| luxR and HSL regulated promoter|| 96 bp|| Verified|| Yes
 |-
-|BBa_I0466|| RhlR protein generator|| 983 bp|| Verified
+|BBa_I0466|| RhlR protein generator|| 983 bp|| Verified|| Yes
 |-
-|BBa_I0407|| Produces lasI and outputs ECFP|| 1674 bp|| Verified
+|BBa_I0407|| Produces lasI and outputs ECFP|| 1674 bp|| Verified|| Yes
 |-
-|BBa_R0079|| LasR and PAI regulated promoter || 198 bp|| Verified
+|BBa_R0079|| LasR and PAI regulated promoter || 198 bp|| Verified|| Yes
 |-
 |BBa_R0063|| LuxR and HSL regulated promoter|| 192 bp|| No successful Mini-Preps
 |-
-|BBa_K09100|| Receiver for AHL and outputs GFP|| 1987 bp|| Verified
+|BBa_K09100|| Receiver for AHL and outputs GFP|| 1987 bp|| Verified|| Yes
 |-
 |BBa_J04450|| RFP coding device switched on by IPTG|| 2110 bp|| Much smaller on gel
 |-
 |}
 -We will be sending all of these parts to be sequenced <br>

@@ Line 12: / Line 12: @@
 !width="65"|Size of Insert (w/ BioBrick ends)
 !width="150"|Verification using Gel Electrophoresis
 |-
-|BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102 bp||Appears to be reversed with J04430
+|BBa_F2620|| Accepts 3OC6HSL as an input and outputs PoPS||1102 bp||Appears to be reversed with J04430|| Yes
 |-
-|BBa_J04430|| GFP generator switched on by IPTG||1124 bp|| Appears to be reversed with F2620
+|BBa_J04430|| GFP generator switched on by IPTG||1124 bp|| Appears to be reversed with F2620|| Yes
 |-
-|BBa_P0440|| Produces tetR||881 bp|| Verified
+|BBa_P0440|| Produces tetR||881 bp|| Verified|| Yes
 |-
-|BBa_E0240|| GFP generator|| 917 bp||Verified
+|BBa_E0240|| GFP generator|| 917 bp||Verified|| Yes
 |-
-|BBa_I132623|| Lux receiver that produces luxR and outputs EYFP|| 2085 bp|| Much smaller on gel
+|BBa_I132623|| Lux receiver that produces luxR and outputs EYFP|| 2085 bp|| Much smaller on gel ||Yes
 |-
-|BBa_I0426|| Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP ||2839 bp|| Verified
+|BBa_I0426|| Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP ||2839 bp|| Verified|| Yes
 |-
-|BBa_C0061|| Produces luxI (w/ LVA tag) ||659 bp|| Verified
+|BBa_C0061|| Produces luxI (w/ LVA tag) ||659 bp|| Verified|| Yes
 |-
 |BBa_C0261|| Produces luxI (w/ RBS)|| 702 bp|| No successful Mini-Preps
@@ Line 107: / Line 108: @@
 |-
 |} <br>
 [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']

@@ Line 132: / Line 132: @@
 |F2620|| Accepts 30C6HSL as an input and outputs PoPS
 |-
+|J04450|| RFP coding device switched on by IPTG
 |}
 [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']

@@ Line 118: / Line 118: @@
 |P0440|| Produces tetR
 |-
 |}
 [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']

@@ Line 46: / Line 46: @@
 -We will be sending all of these parts to be sequenced <br>
 <br>
 '''Gels used to verify parts''' <br>
 ''Gel A'' <br>
@@ Line 106: / Line 107: @@
 |-
 |} <br>
+'''Parts we have Glycerol Stocks for'''
 [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates''']