Test pLsrR/cI - Revision history

XiZhu at 14:05, 1 July 2008

2008-07-01T14:05:57Z

XiZhu at 14:05, 1 July 2008

2008-07-01T14:05:26Z

XiZhu at 14:04, 1 July 2008

2008-07-01T14:04:39Z

XiZhu at 13:53, 1 July 2008

2008-07-01T13:53:36Z

AnGordon at 19:51, 30 June 2008

2008-06-30T19:51:03Z

New page

pLsrR/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.

Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP does not glow

add AI-2

Some promoter→LsrK+LsrR+t+t+pLsrR/cI→GFP glows

add AI-2

Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glows

{| border="1" cellpadding="2"
!width="30"|LsrR
!width="30"|cI
!width="30"|AI-2 + GFP
!width="30"|AI-2 - GFP
|-
| 0 || 0 || 1 || 1
|-
| 1 || 0 || 1 || 0
|-
| 0 || 1 || 0 || 0
|-
| 1 || 1 || 0 || 0
|}

@@ Line 1: / Line 1: @@
 pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
-)Test LsrA repression.
+'''1)Test LsrA repression.'''
 Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP   does not glow
@@ Line 13: / Line 13: @@
 .         B0034+K091001+B0015+K091106→E0240
 )Test cI repression.
 Some promoter→RBS+cI+TT+pLsrA/cI→GFP   does not glow
@@ Line 25: / Line 26: @@
 .        B0034+C0051+B0015+K091106→E0240
 )Test LsrA and cI repression.
 Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
@@ Line 38: / Line 40: @@
-)Control.
+'''4)Control.'''
 pLsrR/cI→GFP glows

@@ Line 3: / Line 3: @@
 )Test LsrA repression.
-Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP   does not glow
+Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP   does not glow
 add AI-2
-Some promoter→RBS+LsrR+t+t+pLsrA/cI→GFP glows
+Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP glows
-−
 )Test cI repression.
-Some promoter→RBS+cI+t+t+pLsrA/cI→GFP   does not glow
+Some promoter→RBS+cI+TT+pLsrA/cI→GFP   does not glow
 add cI
-Some promoter→RBS+cI+t+t+pLsrA/cI→GFP   does not glow
+Some promoter→RBS+cI+TT+pLsrA/cI→GFP   does not glow
-−
 )Test LsrA and cI repression.
-Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow
+Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
 add AI-2
-Some promoter→LsrK+LsrR+cI+t+t+pLsrR/cI→GFP does not glow
+Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
 )Control.
 pLsrR/cI→GFP glows
 {| border="1" cellpadding="2"

@@ Line 52: / Line 52: @@
 !width="30"|AI-2 + GFP
 !width="30"|AI-2 - GFP
 |-
 | 1 || 0 || 1 || 0
@@ Line 60: / Line 58: @@
 |-
 | 1 || 1 || 0 || 0
 |}