What to do with a new clone - Revision history

Eckdahl at 14:17, 1 October 2011

2011-10-01T14:17:47Z

Macampbell at 20:07, 13 May 2011

2011-05-13T20:07:09Z

Macampbell at 19:57, 13 May 2011

2011-05-13T19:57:02Z

Eckdahl at 16:26, 1 June 2010

2010-06-01T16:26:17Z

Eckdahl at 16:25, 1 June 2010

2010-06-01T16:25:31Z

Eckdahl at 16:23, 1 June 2010

2010-06-01T16:23:59Z

Eckdahl at 16:23, 1 June 2010

2010-06-01T16:23:48Z

Eckdahl at 16:23, 1 June 2010

2010-06-01T16:23:23Z

New page

Yay! You made a new clone. Good work. Here are the several important steps you need to take next.

'''Register the Clone in the Registry of Standard Biological Parts'''

#Go to the Registry (http://partsregistry.org/Main_Page) and choose "Add a part"
# Decide whether the new part is a '''Basic Part''' (discrete function units that cannot be subdivided, like promoter, RBS, coding sequence), '''Composite Part''' (functional unit composed of basic parts, like gene expression cassette, inverter), or '''Construction Intermediate''' (no function, byproduct of construction).
#Choose the number for the next available part
#Be sure to include short and long descriptions of the part
#Once data is available on the part, go back and include it as "Experience"

'''Enter the Clone into GCAT-alog'''

# Enter the information for each frozen tube into our [http://gcat.davidson.edu/GCATalog-r2/GCATalog.htm online freezer stock database]. It is '''VERY IMPORTANT''' to enter each tube into the database, even duplicates. We have too many tubes now and they are hard to locate.

'''Make a Glycerol Stock'''

In order to preserve your new clone for use by others for years to come, you need to make a glycerol stock. Do this right away. Bacteria on plates stored at 4 degrees survive for about 1 month. Bacteria in liquid cultures survive about 2 days on the bench and about 1 week at 4 degrees. Plasmids degrade rapidly in cells left in liquid culture.

# Grow a fresh overnight culture of the clone in the appropriate media with antibiotic
# Get a cryotube, cap, and colored cap insert. Write the part number on the color cap insert. Write the part number, the plasmid type (pSB1A2, pSB4T3, etc.), and a short description on the tube.
# Transfer 300 ul of glycerol into the labeled cryotubes.
# Add 700 µL of the overnight bacterial culture. Put the cap on and shake VERY vigorously, or vortex vigorously.
# Put the tube in the numbered freezer box location that was determined by GCAT-alog.
# When you want to retrieve some cells from the glycerol stocks, do not let them thaw. Use a sterile toothpick and scrape off some of the ice and put this onto your plate or into a liquid culture with the appropriate antibiotic.

'''Put some of the Plasmid in the Current iGEM Box'''

#Label a 1.5 ml tube with the part number, plasmid, and a short description of the part
#Place 10 ul of a miniprep of the plasmid into the tube. The concentration should be at least 40 ng/ul
#Place the tube in the freezer box labeled with the current year and "iGEM Registry Clones"

@@ Line 12: / Line 12: @@
 '''Enter the Clone into GCAT-alog'''
-# Enter the information for each frozen tube into our online freezer stock database called [http://gcat.davidson.edu/GCATalog-r2/GCATalog.htm GCAT-alog].
+# Enter the information for each frozen tube into our online freezer stock database called [http://gcat.davidson.edu/GCATalog/ GCAT-alog].
 # Be sure to include all the requested information
 <br>
 '''Make a Glycerol Stock'''
-In order to preserve your new clone for use by others for years to come, you need to make a glycerol stock. Do this right away.  Bacteria on plates stored at 4 degrees survive for about 1 month. Bacteria in liquid cultures survive about 2 days on the bench and about 1 week at 4 degrees. Plasmids degrade rapidly in cells left in liquid culture.
+In order to preserve your new clone for use by others for years to come, you need to make a glycerol stock (see: [[Glycerolstocks_How_to_Make_Glycerol_Stocks_of_Bacteria ]]). Do this right away.  Bacteria on plates stored at 4 degrees survive for about 1 month. Bacteria in liquid cultures survive about 2 days on the bench and about 1 week at 4 degrees. Plasmids degrade rapidly in cells left in liquid culture.
 # Grow a fresh overnight culture of the clone in the appropriate media with antibiotic

@@ Line 4: / Line 4: @@
 '''Register the Clone in the Registry of Standard Biological Parts'''
-#Go to the [http://ung.igem.org/Lab:Campbell_M_Lab Campbell Lab Wiki ]and choose "Add a part"
+#Go to the [http://partsregistry.org/Main_Page Registry]and choose "Add a part"
 # Decide whether the new part is a '''Basic Part''' (discrete function units that cannot be subdivided, like promoter, RBS, coding sequence), '''Composite Part''' (functional unit composed of basic parts, like gene expression cassette, inverter), or '''Construction Intermediate''' (no function, byproduct of construction).
 #Choose the number for the next available part

@@ Line 1: / Line 1: @@
 Yay! You made a new clone.  Good work.  Here are the several important steps you need to take next.
 <br>
 '''Register the Clone in the Registry of Standard Biological Parts'''

@@ Line 1: / Line 1: @@
 Yay! You made a new clone.  Good work.  Here are the several important steps you need to take next.
+<br>
 '''Register the Clone in the Registry of Standard Biological Parts'''
@@ Line 8: / Line 8: @@
 #Be sure to include short and long descriptions of the part
 #Once data is available on the part, go back and include it as "Experience"
+<br>
 '''Enter the Clone into GCAT-alog'''
 # Enter the information for each frozen tube into our [http://gcat.davidson.edu/GCATalog-r2/GCATalog.htm online freezer stock database]. It is '''VERY IMPORTANT''' to enter each tube into the database, even duplicates. We have too many tubes now and they are hard to locate.
+<br>
 '''Make a Glycerol Stock'''
@@ Line 23: / Line 23: @@
 # Put the tube in the numbered freezer box location that was determined by GCAT-alog.
 # When you want to retrieve some cells from the glycerol stocks, do not let them thaw. Use a sterile toothpick and scrape off some of the ice and put this onto your plate or into a liquid culture with the appropriate antibiotic.
+<br>
 '''Put some of the Plasmid in the Current iGEM Box'''