Difference between revisions of "Burkholderia phage Virus Hit from Spacer 14 in Crispr One: Blast Alignment to Other Halophiles"
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| − | Accession Number of this Virus= EU307292.1 | + | Accession Number of this Virus= EU307292.1- only partial sequence!!!!!!! thats just what we have |
| − | What species does this virus infect? Blastn against nr/nt database. . . | + | '''What species does this virus infect'''? Blastn against nr/nt database. . . |
| − | + | [[Image:virus2.jpg]] | |
| − | + | Our species does not show up because different parameters for short sequence when we blasted the spacer | |
| − | |||
| − | Blasted against other halophiles to determine if other halophiles have segments of this viral genome in its spacers and genome in general. . . | + | |
| + | Blasted our species and got some hits but no hits of spacers sharing similarity with this virus. . . that is because of the different parameters?????????? | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | '''Blasted against other halophiles''' to determine if other halophiles have segments of this viral genome in its spacers and genome in general. . . | ||
| + | |||
| + | Halorhabdus utahensis: Accession number determined from NCBI: NC_013158.1 | ||
| + | |||
| + | CONCLUSION: these viral hits are not in spacers. . . this virus did not enter spacer in this species | ||
| + | |||
| + | [[Image:Virus2HU.jpg]] | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | Halobacterium salinarium R1: Accession number determined from NCBI: NC_010364 | ||
| + | |||
| + | [[Image:virus2HS.jpg]] | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | Haloarcula vallismortis | ||
| + | |||
| + | [[Image:virus2HV1.jpg]] | ||
| + | |||
| + | [[Image:virus2HV2.jpg]] | ||
| + | |||
| + | [[Image:virus2HV3.jpg]] | ||
| + | |||
| + | [[Image:virus2HV4.jpg]] | ||
| + | |||
| + | [[Image:virus2HV5.jpg]] | ||
| + | |||
| + | [[Image:virus2HV6.jpg]] | ||
| + | |||
| + | [[Image:virus2HV7.jpg]] | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | Haloarcula sinaiiensis | ||
| + | |||
| + | CONCLUSION: this virus did not insert into the spacers of this species | ||
| + | |||
| + | [[Image:virus2H1.jpg]] | ||
| + | |||
| + | [[Image:virus2H2.jpg]] | ||
| + | |||
| + | [[Image:virus2H3.jpg]] | ||
| + | |||
| + | [[Image:virus2H4.jpg]] | ||
| + | |||
| + | [[Image:virus2H5.jpg]] | ||
| + | |||
| + | [[Image:virus2H6.jpg]] | ||
Latest revision as of 05:28, 18 November 2009
Accession Number of this Virus= EU307292.1- only partial sequence!!!!!!! thats just what we have
What species does this virus infect? Blastn against nr/nt database. . .
Our species does not show up because different parameters for short sequence when we blasted the spacer
Blasted our species and got some hits but no hits of spacers sharing similarity with this virus. . . that is because of the different parameters??????????
Blasted against other halophiles to determine if other halophiles have segments of this viral genome in its spacers and genome in general. . .
Halorhabdus utahensis: Accession number determined from NCBI: NC_013158.1
CONCLUSION: these viral hits are not in spacers. . . this virus did not enter spacer in this species
Halobacterium salinarium R1: Accession number determined from NCBI: NC_010364
Haloarcula vallismortis
Haloarcula sinaiiensis
CONCLUSION: this virus did not insert into the spacers of this species
