Difference between revisions of "Prepare PCR product for sequencing"
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Revision as of 21:43, 6 July 2012
You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.
- Pipet 5 µL of clean PCR product into the bar coded tube.
- Pipet 9 µL of sequencing primer into the same bar coded tube.
- Place the small cap back onto the bar coded sequencing tube.
- Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
