Difference between revisions of "J-GGA Scaffold Sequences"
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Revision as of 18:42, 31 May 2013
J-GGA Scaffold Oligos
Prefix: Gaattcgcggccgcttctagag
Junction A: Atagtctgttcatggtgc
Spacer: acgt
Junction B: Tattgatgtagttaaggc
Spacer: cgga
Junction C: Tttctaatggggaccatc
Spacer: ttag
Junction D: Ttaagctatgtattcctg
Suffix: tactagtagcggccgctgcag
Top Strand
SCAFF_TOP1; AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGG
SCAFF_TOP2; GGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA
BOTTOM STRAND (5’ TO 3’)
SCAFF_BOT1; GCGGCCGCTACTAGTACAGGAATACATAGCTTAACTAAGATGGTCCCCATTAGAAATCCGGCCTTAACTAC
SCAFF_BOT2; ATCAATAACGTGCACCATGAACAGACTATCTCTAGAAGCGGCCGCG
Annealed
AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGGGGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA
GCGCCGGCGAAGATCTCTATCAGACAAGTACCACGTGCAATAACTACATCAATTCCGGCCTAAAGATTACCCCTGGTAGAATCAATTCGATACATAAGGACATGATCATCGCCGGCG
