Difference between revisions of "Origin of Replication"
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This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation. | This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation. | ||
| + | |||
| + | ---- | ||
| + | '''RNA I/RNA II Literature''' | ||
| + | |||
| + | Replication and Control of Circular Bacterial Plasmids | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/pdf/mr000434.pdf] | ||
| + | |||
| + | Plasmid ColE1 Incompatibility Determined by Interaction of RNA I | ||
| + | with Primer Transcript | ||
| + | [http://www.pnas.org/content/78/10/6096.full.pdf] | ||
| + | |||
| + | Formation of an RNA Primer for Initiation of Replication of ColE1 | ||
| + | DNA by ribonuclease H | ||
| + | [http://www.pnas.org/content/77/5/2450.full.pdf] | ||
| + | |||
| + | Control of pMB1 Replication: Inhibition of Primer Formation | ||
| + | by Rop Requires RNA1 | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557524/pdf/emboj00310-0150.pdf] | ||
| + | |||
| + | High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II. | ||
| + | [http://content.ebscohost.com/pdf9/pdf/1992/ESL/15Nov92/15903115.pdf?T=P&P=AN&K=15903115&S=R&D=eih&EbscoContent=dGJyMMTo50SeprU4yOvsOLCmr0uep65Ssa24SLSWxWXS&ContentCustomer=dGJyMK6nr0W2pq5T69fnhrnb5ofx6gAA] | ||
| + | |||
| + | Control of ColE1 Replication: Low Affinity Specific Binding | ||
| + | of Rop (Rom) to RNAI and RNAII | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC454354/pdf/emboj00139-0256.pdf] | ||
| + | |||
| + | Plasmid pT181 Replication is Regulated by Two Countertranscripts | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC397100/pdf/pnas00343-0012.pdf] | ||
| + | |||
| + | The mutH Gene Regulates the Replication and Methylation | ||
| + | of the pMB1 Origin | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207916/pdf/jbacter00100-0187.pdf] | ||
| + | |||
| + | Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking | ||
| + | the Tetracycline Resistance Gene | ||
| + | [http://www.microbiology.ubc.ca/sites/default/files/roles/drupal_ungrad/JEMI/10/10-18.pdf] | ||
| + | |||
| + | '''Quantitative PCR Literature''' | ||
| + | |||
| + | Improved Determination of Plasmid Copy Number Using Quantitative Real-Time PCR for Monitoring Fermentation Processes | ||
| + | [http://www.microbialcellfactories.com/content/7/1/6] | ||
| + | |||
| + | Fast SYBR Green Master Mix Protocol | ||
| + | [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_046776.pdf] | ||
| + | ---- | ||
| + | '''[[Oligos and Primers]]''' | ||
Latest revision as of 15:04, 17 June 2013
RNA I and RNA II gene and promoter sites in pSB1a2-BR
File:PSB1A2 (pMB1)- RNA 1 and RNA 2 sites.docx
This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.
RNA I/RNA II Literature
Replication and Control of Circular Bacterial Plasmids [1]
Plasmid ColE1 Incompatibility Determined by Interaction of RNA I with Primer Transcript [2]
Formation of an RNA Primer for Initiation of Replication of ColE1 DNA by ribonuclease H [3]
Control of pMB1 Replication: Inhibition of Primer Formation by Rop Requires RNA1 [4]
High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II. [5]
Control of ColE1 Replication: Low Affinity Specific Binding of Rop (Rom) to RNAI and RNAII [6]
Plasmid pT181 Replication is Regulated by Two Countertranscripts [7]
The mutH Gene Regulates the Replication and Methylation of the pMB1 Origin [8]
Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking the Tetracycline Resistance Gene [9]
Quantitative PCR Literature
Improved Determination of Plasmid Copy Number Using Quantitative Real-Time PCR for Monitoring Fermentation Processes [10]
Fast SYBR Green Master Mix Protocol [11]
