Difference between revisions of "Team 1's Brainstorm for Future Programmed Evolution Research"
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- Any other metabolic improvements to caffeine -> theophylline pathway | - Any other metabolic improvements to caffeine -> theophylline pathway | ||
| − | - Stopping the pathway converting | + | - Stopping the pathway converting ammeline (guanine deamylase), seeing whether that pathway is absolutely necessary to cell survival. (In the melamine -> ammeline -> … pathway) |
- What else besides theophylline? (MWSU “laundry list”) | - What else besides theophylline? (MWSU “laundry list”) | ||
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Riboswitch / RBS combo that is a single modular structure (C-Dog?) | Riboswitch / RBS combo that is a single modular structure (C-Dog?) | ||
| − | Finding a riboswitch that works with | + | Finding a riboswitch that works with ammeline |
==Our picks== | ==Our picks== | ||
| − | + | <strike>Testing the ThyA fitness module developed at Davidson, or finding other ways to implement it.</strike> (Covered by second team.) | |
| − | + | Investigating riboswitch-RBS interactions - The problem is that the function of promoters and RBS's are context-dependent, based on each other and on the gene of interest. C-dog is more reliable, but might be improved. The goal of investigating the riboswitch-RBS interactions would be to develop a hybrid riboswitch/C-dog combo, making the riboswitch more reliable. | |
| − | + | Testing a new metabolic pathway - choose the pathway based on which riboswitches are already known | |
| − | |||
| − | Testing a new metabolic pathway | ||
Latest revision as of 20:28, 26 May 2014
Barcode of 7 bp (7^4 combinations)
- Use GGA . . . still have to work out restriction enzyme, other details
Changing internal (genetic) elements…riboswitch, other RBS and origins and chaperones
- …Using new ones
- …introducing mutations to improve them
Multistep/more complex pathways?
- Any other metabolic improvements to caffeine -> theophylline pathway
- Stopping the pathway converting ammeline (guanine deamylase), seeing whether that pathway is absolutely necessary to cell survival. (In the melamine -> ammeline -> … pathway)
- What else besides theophylline? (MWSU “laundry list”)
New fitness modules
- Testing /improving /adapting the ThyA fitness module
- Building new fitness modules
New riboswitches
Trying new riboswitch that interacts with coenzyme B12
Building a riboswitch
- …Using/improving Catherine Doyle’s riboswitch builder, which generates potential riboswitch sequences based on a given aptamer sequence
Riboswitch / RBS combo that is a single modular structure (C-Dog?)
Finding a riboswitch that works with ammeline
Our picks
Testing the ThyA fitness module developed at Davidson, or finding other ways to implement it. (Covered by second team.)
Investigating riboswitch-RBS interactions - The problem is that the function of promoters and RBS's are context-dependent, based on each other and on the gene of interest. C-dog is more reliable, but might be improved. The goal of investigating the riboswitch-RBS interactions would be to develop a hybrid riboswitch/C-dog combo, making the riboswitch more reliable.
Testing a new metabolic pathway - choose the pathway based on which riboswitches are already known
