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| | + | [[May 27, 2014]] |
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| − | == To Do ==
| + | [[May 28, 2014]] |
| − | 1) Get melamine deaminase in correct location in the plasmid.
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| − | 2) Retest riboswitches with the biosensor.
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| − | 3) Test riboswitches in ThyA fitness module (insert ThyA- into plasmid).
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| − | 4) Find a way to better dissolve ammeline (e.g. instead of toxic NaOH, use DMSO).
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| − | 5) Research for more riboswitch options.
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| − | 6) Use UNAFold and futher study of Dixon article to create new riboswitches.
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| − | 7) Use pLac promoter instead of P5.
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| − | 8) Put it all together: melamine deaminase, riboswitch, etc. This allows for the testing of different promoters and ribosome binding sites to optimize the fitness module.
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