Difference between revisions of "New Chaperone PCR"
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(Created page with " == Preparation == 1. 2uL Bacteria 2. 0.5uL New Chaperone Mix 3. 7.5uL dH20 4. 10uL GoTac Green == Programed PCR Protocol == Stage 1: 94ºC 10 minutes Stage 2: 20 cycles...") |
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1. 2uL Bacteria | 1. 2uL Bacteria | ||
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2. 0.5uL New Chaperone Mix | 2. 0.5uL New Chaperone Mix | ||
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3. 7.5uL dH20 | 3. 7.5uL dH20 | ||
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4. 10uL GoTac Green | 4. 10uL GoTac Green | ||
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== Programed PCR Protocol == | == Programed PCR Protocol == | ||
| − | Stage 1: | + | '''Stage 1:''' |
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94ºC 10 minutes | 94ºC 10 minutes | ||
| − | Stage 2: 20 cycles | + | '''Stage 2:''' |
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| + | 20 cycles | ||
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94ºC 15 seconds | 94ºC 15 seconds | ||
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51ºC 15 seconds | 51ºC 15 seconds | ||
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74ºC 2 minutes | 74ºC 2 minutes | ||
| − | Stage 3: | + | '''Stage 3:''' |
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74ºC 5 minutes | 74ºC 5 minutes | ||
Latest revision as of 19:10, 28 May 2014
Preparation
1. 2uL Bacteria
2. 0.5uL New Chaperone Mix
3. 7.5uL dH20
4. 10uL GoTac Green
Programed PCR Protocol
Stage 1:
94ºC 10 minutes
Stage 2:
20 cycles
94ºC 15 seconds
51ºC 15 seconds
74ºC 2 minutes
Stage 3:
74ºC 5 minutes
