Difference between revisions of "January 21, 2016"
From GcatWiki
| Line 5: | Line 5: | ||
*Researchers clustered genes with similar trends in differential gene expression together. | *Researchers clustered genes with similar trends in differential gene expression together. | ||
*STEM is a source of gene identifiers and clusters. | *STEM is a source of gene identifiers and clusters. | ||
| − | *Volcano plots showed significant difference for no change in gene expression levels. | + | *Volcano plots showed significant difference for no change in gene expression levels. |
| + | |||
| + | |||
| + | |||
=== Questions to Consider === | === Questions to Consider === | ||
*What are the names of the genes the researchers clustered? | *What are the names of the genes the researchers clustered? | ||
| − | + | **Investigate ncbi gene bank | |
*What started the cascade? | *What started the cascade? | ||
| − | + | **If known '''''transcription factors''''' activate genes in the cascade, maybe we can find them early on. | |
*If a few genes don't change, is that biologically significant? | *If a few genes don't change, is that biologically significant? | ||
| − | |||
| Line 27: | Line 29: | ||
*Very few "N's." A "N" indicates that RNAseq was unable to pick a base. | *Very few "N's." A "N" indicates that RNAseq was unable to pick a base. | ||
*Intestine has smooth GC curve line. | *Intestine has smooth GC curve line. | ||
| − | *The class was unable to determine what exactly "Sequence Duplication Levels" showed us. | + | *The class was unable to determine what exactly "Sequence Duplication Levels" showed us. |
| + | *Overall, our data is of good quality. | ||
| − | ''''' | + | '''''Moving forward, we need to: |
| − | + | *Trim off the first 4 bases. | |
| − | + | *Discard reads with a score less than 15. | |
| − | + | *Name all the reads. | |
| − | + | *Sort all the reads. | |
| − | + | *Exhaust publicly avaible data sets.''''' | |
| − | ''INCLUDE SCREENSHOTS OF DATA WHEN YOU FIGURE OUT HOW TO UPLOAD PICTURES'' | + | |
| + | |||
| + | ''INCLUDE SCREENSHOTS OF DATA WHEN YOU FIGURE OUT HOW TO UPLOAD PICTURES'' | ||
| − | |||
| + | [http://gcat.davidson.edu/mediawiki-1.19.1/index.php/Ashlyn Ashlyn's Main Page] | ||
Revision as of 14:51, 13 February 2016
Contents
[hide]Article Discussion
"The Burmese Pythod Genome Reveals the Molecular Basis for Extreme Adaptation in Snakes"
- Researchers clustered genes with similar trends in differential gene expression together.
- STEM is a source of gene identifiers and clusters.
- Volcano plots showed significant difference for no change in gene expression levels.
Questions to Consider
- What are the names of the genes the researchers clustered?
- Investigate ncbi gene bank
- What started the cascade?
- If known transcription factors activate genes in the cascade, maybe we can find them early on.
- If a few genes don't change, is that biologically significant?
Classwork
Analyze FastQC data analysis results downloaded from class on January 19, 2016
- A quality score above 40 is good.
- Sequence codes match the reagents provided in class document.
- All reads are 76bp.
- Very few "N's." A "N" indicates that RNAseq was unable to pick a base.
- Intestine has smooth GC curve line.
- The class was unable to determine what exactly "Sequence Duplication Levels" showed us.
- Overall, our data is of good quality.
Moving forward, we need to:
- Trim off the first 4 bases.
- Discard reads with a score less than 15.
- Name all the reads.
- Sort all the reads.
- Exhaust publicly avaible data sets.
INCLUDE SCREENSHOTS OF DATA WHEN YOU FIGURE OUT HOW TO UPLOAD PICTURES
References
- Castoe, Todd A., et al. “The Burmese python genome reveals the molecular basis for extreme adaptation in snakes.” Pnas.org. (2013)
