Difference between revisions of "Pouring an Agarose Gel"

Latest revision as of 19:03, 15 February 2017

Pouring an Agarose Gel

1. Add 35 mL of 1X TAE buffer to a 125 ml flask
2. Add agarose (0.35 grams agarose for 1% gel).
3. Microwave for 50 seconds
4. Swirl and microwave for 5 seconds - repeat 3 times
5. Place flask in 55 C water bath to cool
6. Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
7. Swirl to mix and pour into gel tray with comb