Difference between revisions of "DNA Sequencing at Eurofins"
From GcatWiki
(Created page with "# Prepare template and primer solutions at the concentrations listed at the bottom of this page. # Grab the appropriate number of bar-code tubes to be sent off for sequencing....") |
|||
| Line 1: | Line 1: | ||
| − | + | Prepare template and primer solutions at the concentrations listed at the bottom of this page. | |
| + | |||
# Grab the appropriate number of bar-code tubes to be sent off for sequencing. | # Grab the appropriate number of bar-code tubes to be sent off for sequencing. | ||
# Record the code on each tube and what clone will be in it inside your lab notebook. | # Record the code on each tube and what clone will be in it inside your lab notebook. | ||
| − | # | + | # Add your sample to the correct bar-code tube. |
| + | |||
| + | '''How to Prepare''' | ||
| + | |||
| + | 1.Prepare aqueous template and primer solution at the concentrations listed below. | ||
| + | |||
| + | 2. Add 8ul template + 4ul (2uM) to each tube by inserting your pipet tip through the split seal (total volume = 12ul) | ||
| + | |||
| + | 3. Place tubes into a Blue Bag or other ziplock bag | ||
| + | |||
| + | 4. Ship using a UPS padded envelope from the Overnight Kit or place Blue Bag in a near-by Eurofins Genomics Dropbox | ||
| + | |||
| + | PCR < 300 Bp (10-20ng/ul) | ||
| + | |||
| + | PCR 300-1000 Bp (20-40ng/ul) | ||
| + | |||
| + | PCR >1000 Bp (40-60ng/ul) | ||
| + | |||
| + | Plasmids all lenghts (100-200ng/ul) | ||
Latest revision as of 15:00, 21 June 2018
Prepare template and primer solutions at the concentrations listed at the bottom of this page.
- Grab the appropriate number of bar-code tubes to be sent off for sequencing.
- Record the code on each tube and what clone will be in it inside your lab notebook.
- Add your sample to the correct bar-code tube.
How to Prepare
1.Prepare aqueous template and primer solution at the concentrations listed below.
2. Add 8ul template + 4ul (2uM) to each tube by inserting your pipet tip through the split seal (total volume = 12ul)
3. Place tubes into a Blue Bag or other ziplock bag
4. Ship using a UPS padded envelope from the Overnight Kit or place Blue Bag in a near-by Eurofins Genomics Dropbox
PCR < 300 Bp (10-20ng/ul)
PCR 300-1000 Bp (20-40ng/ul)
PCR >1000 Bp (40-60ng/ul)
Plasmids all lenghts (100-200ng/ul)
