Difference between revisions of "Status Of Building Parts"
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[[Image:gelA.jpg]]<br> | [[Image:gelA.jpg]]<br> | ||
These are the parts run on a 0.5% gel.<br> | These are the parts run on a 0.5% gel.<br> | ||
| + | [[Image:gelB.jpg]]<br> | ||
| + | These parts were run on a 2.5% gel.<br> | ||
[http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates'''] | [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates'''] | ||
Revision as of 18:53, 5 June 2008
MWSU + Davidson Status of Building Sender and Receiver
plux-lac hybrid promoter:
- I751502
- In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI
Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha
Parts we have Mini-Preps and Digestions for
| Part | Description | Size of Insert (w/ BioBrick ends) | Verification using PCR |
|---|---|---|---|
| BBa_F2620 | Accepts 3OC6HSL as an input and outputs PoPS | 1102 bp | Appears to be reversed with J04430 |
| BBa_J04430 | GFP generator switched on by IPTG | 1124 bp | Appears to be reversed with F2620 |
| BBa_P0440 | Produces tetR | 881 bp | Verified |
| BBa_E0240 | GFP generator | 917 bp | Verified |
| BBa_I132623 | Lux receiver that produces luxR and outputs EYFP | 2085 bp | Much smaller on gel |
| BBa_I0426 | 2839 bp | Verified | |
| BBa_C0061 | Produces luxI (w/ LVA tag) | 659 bp | Verified |
| BBa_C0261 | Produces luxI (w/ RBS) | 702 bp | No successful Mini-Preps |
| BBa_R0062 | luxR and HSL regulated promoter | 96 bp | Verified |
| BBa_I0466 | RhlR protein generator | 983 bp | Verified |
| BBa_I0407 | Produces lasI and outputs ECFP | 1674 bp | Verified |
| BBa_R0079 | LasR and PAI regulated promoter | 198 bp | Verified |
| BBa_R0063 | LuxR and HSL regulated promoter | 192 bp | Have not yet digested |
| BBa_K09100 | Receiver for AHL and outputs GFP | 1987 bp | Verified |
| BBa_J04450 | RFP coding device switched on by IPTG | 2110 bp | Much smaller on gel |
These are the parts run on a 0.5% gel.
These parts were run on a 2.5% gel.
Look at USTC 2007 for logic gates
T9002 - no colonies
F2622 + E0240 (ligated/colonies coming up)
+ 
S03632 (ligations needed)
But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
+ 
+ 
R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
+ 
F2620 + E0240 (minis growing/ frag available)
+
