Difference between revisions of "LasR/cI"
From GcatWiki
| Line 1: | Line 1: | ||
| − | + | '''Goal:''' | |
| − | I13453+B0034+C0079+ | + | '''pBAD+RBS+lasR+TT'''(James DC) |
| + | |||
| + | '''S0388?'''(I13453+B0034+C0079+B0015) | ||
| + | |||
| + | '''pLasR/cI+RBS+LuxI+RBS+Mnt+TT''' | ||
| + | |||
| + | '''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015''' | ||
| + | |||
| + | '''Ligation Plan''' | ||
| + | {| border="1" cellpadding="2" | ||
| + | !width="20"| | ||
| + | !width="30"|Vector | ||
| + | !width="110"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"|Insert | ||
| + | !width="110"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"|Product | ||
| + | !width="30"| | ||
| + | |- | ||
| + | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
| + | |- | ||
| + | | 1|| K09110? ||plasR/cI ||R/X; S/P||S/P || 215+2079||C0261||RBS+luxI ||R/X;S/P ||X/P ||661 ||TBD || | ||
| + | |- | ||
| + | | 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD || | ||
| + | |- | ||
| + | | 3||TBD||RBS+Mnt+TT ||R/X;S/P ||R/X || ||TBD||plasR/cI+RBS+luxI ||R/X; S/P ||R/S || ||TBD || | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | '''Parts''' | ||
| + | {| border="1" cellpadding="2" | ||
| + | !width="40"|BBa_ | ||
| + | !width="110"|Description | ||
| + | !width="60"|Source | ||
| + | !width="100"|location('07) | ||
| + | !width="35"|Vector | ||
| + | !width="40"|Responsible | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_K09110? K09110?] ||plasR/cI ||MW ||N/A ||pSB1A2 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || | ||
| + | |- | ||
| + | |} | ||
Revision as of 15:57, 10 July 2008
Goal:
pBAD+RBS+lasR+TT(James DC)
S0388?(I13453+B0034+C0079+B0015)
pLasR/cI+RBS+LuxI+RBS+Mnt+TT
K09110?+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | K09110? | plasR/cI | R/X; S/P | S/P | 215+2079 | C0261 | RBS+luxI | R/X;S/P | X/P | 661 | TBD | |
| 2 | B0015 | TT | R/X; S/P | R/X | 129+2079 | I13025 | RBS+Mnt | R/X;S/P | R/S | 331 | TBD | |
| 3 | TBD | RBS+Mnt+TT | R/X;S/P | R/X | TBD | plasR/cI+RBS+luxI | R/X; S/P | R/S | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K09110? | plasR/cI | MW | N/A | pSB1A2 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 |
