Difference between revisions of "Parts needed for XOR"

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|[http://partsregistry.org/Part:BBa_C0061 BBa_C0061]||. LuxI w/ LVA ||Plt.1-5O; pSB1A2 ||amp ||  ||  
 
|[http://partsregistry.org/Part:BBa_C0061 BBa_C0061]||. LuxI w/ LVA ||Plt.1-5O; pSB1A2 ||amp ||  ||  
 
|-
 
|-
−
|BBa_|| ||  ||  ||  ||  
+
|[http://partsregistry.org/Part:pSB4A3 BBa_pSB4A3]|| low copy amp plasmid ||  ||  ||  ||  
 
|-
 
|-
 
|BBa_||  ||  ||  ||  ||   
 
|BBa_||  ||  ||  ||  ||   
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!width="30"|Results  
 
!width="30"|Results  
 
!width="30"|miniprep
 
!width="30"|miniprep
 +
!width="30"|glycerol stock
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091107 BBa_K091107]||. Lux-cI hybrid promoter  ||  || right size ||yes  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091100 BBa_K091100]||. pLac_lux hybrid promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091101 BBa_K091101]||. pTet_Lac hybrid promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091104 BBa_K091104]||. Mnt/LacI Hybrid Promoter  ||  ||  || ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091105 BBa_K091105]||. Mnt/TetR Promoter  ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091106 BBa_K091106]||. LsrA/cI hybrid promoter  ||  ||right size  ||yes  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091001 BBa_K091001]||. LsrR gene ||  ||  ||    ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091002 BBa_K091002]||. LsrK gene ||  ||  ||    ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091109 BBA_K091109]||. LuxS gene ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091110 BBA_K091110]||. LacI promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091111 BBA_K091111]||. LacIQ promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091112 BBA_K091112]||. LacIQ1 promoter ||  ||  ||  ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_K091132 K091132] || pMnt/Tet+RBS+LuxI || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_K091107||Lux-cI hybrid promoter  || || ||  
+
|[http://partsregistry.org/Part:BBa_K091133 K091133] || pBAD+RBS+TetR ||amp || right size ||yes ||yes
 
|-
 
|-
−
|BBa_|| || || ||  
+
|[http://partsregistry.org/Part:BBa_K091137 K091137] || RBS+Mnt+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_|| || || ||
+
|[http://partsregistry.org/Part:BBa_K091144 K091144] || pMnt/Tet+RBS+LuxI+RBS+cI+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_|| || || ||  
+
|[http://partsregistry.org/Part:BBa_K091145 K091145] || pBad+RBS+TetR+TT || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_|| || || ||  
+
|[http://partsregistry.org/Part:BBa_K091147 K091147] || pBAD+RBS+Mnt+TT || amp || right size || yes ||yes
 
|-
 
|-
−
|BBa_|| || || ||
+
|[http://partsregistry.org/Part:BBa_K091160 K091160] || pMnt/Tet+RBS+GFP+TT || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_|| || || ||  
+
| [http://partsregistry.org/Part:BBa_K091175 K091175] || K091145+K091160 || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_|| || || ||    
+
| [http://partsregistry.org/Part:BBa_K091176 K091176] || K091147+K091160 || amp || right size || yes || yes
 
|-
 
|-
−
|BBa_|| || || ||
+
| [http://partsregistry.org/Part:BBa_K091177 K091177] || K091160+K091145 || amp || right size || yes || yes
 +
|-
 +
| [http://partsregistry.org/Part:BBa_K091178 K091178] || K091160+K091147 || amp || right size || yes ||yes
 
|-
 
|-
 
|}
 
|}

Latest revision as of 17:56, 6 August 2008

Basic Parts:

Part Description Location(07 registry) and Vector Resistance Results miniprep
BBa_C0051 . cI w/ LVA Plt.1-5G; pSB1A2 amp
BBa_C0072 . mnt w/ LVA Plt.2-5M; pSB2K3 kan
BBa_C0040 . tetR w/ LVA Plt.1-5C; pSB1A2 amp
BBa_C0062 . LuxR no LVA Plt.1-7A; pSB1A2 amp
BBa_C0061 . LuxI w/ LVA Plt.1-5O; pSB1A2 amp
BBa_pSB4A3 low copy amp plasmid
BBa_
BBa_
BBa_

Composite Parts:

Part Description Location(07 registry) and Vector Resistance Results miniprep
BBa_J06550 . RBS+cI Plt.2-9B; pSB1A2 amp
BBa_S0103 . RBS+cI Plt.1-15C; pSB1A2 amp
BBa_I13025 . RBS+mnt Plt.1-23J; pSB1A2 amp
BBa_S0101 . RBS+tetR Plt.1-13O; pSB1A2 amp
BBa_J37033 . RBS+LuxR(no LVA) Plt.3-8F; pSB1A2 amp
BBa_C0261 . RBS+LuxI Plt.1-24A; pSB1A2 amp
BBa_
BBa_
BBa_

New Parts Constructed:

Part Description Resistance Results miniprep glycerol stock
BBa_K091107 . Lux-cI hybrid promoter right size yes
BBa_K091100 . pLac_lux hybrid promoter
BBa_K091101 . pTet_Lac hybrid promoter
BBa_K091104 . Mnt/LacI Hybrid Promoter
BBa_K091105 . Mnt/TetR Promoter
BBa_K091106 . LsrA/cI hybrid promoter right size yes
BBa_K091001 . LsrR gene
BBa_K091002 . LsrK gene
BBA_K091109 . LuxS gene
BBA_K091110 . LacI promoter
BBA_K091111 . LacIQ promoter
BBA_K091112 . LacIQ1 promoter
K091132 pMnt/Tet+RBS+LuxI amp right size yes yes
K091133 pBAD+RBS+TetR amp right size yes yes
K091137 RBS+Mnt+TT amp right size yes yes
K091144 pMnt/Tet+RBS+LuxI+RBS+cI+TT amp right size yes yes
K091145 pBad+RBS+TetR+TT amp right size yes yes
K091147 pBAD+RBS+Mnt+TT amp right size yes yes
K091160 pMnt/Tet+RBS+GFP+TT amp right size yes yes
K091175 K091145+K091160 amp right size yes yes
K091176 K091147+K091160 amp right size yes yes
K091177 K091160+K091145 amp right size yes yes
K091178 K091160+K091147 amp right size yes yes
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