Difference between revisions of "Will Notebook1"
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==[[User:Wideloache|Wideloache]] 16:20, 10 September 2008 (EDT)== | ==[[User:Wideloache|Wideloache]] 16:20, 10 September 2008 (EDT)== | ||
− | + | On Monday, I got K091136, K091146, and K091117 out of the -80. I need to make pLux' and pLux+Las- and do a genomic insertion of LuxR/LasR before I can test the XOR promoters. | |
=== === | === === | ||
<pre> | <pre> | ||
− | to | + | Begin Primer Synthesis of pLux' and pLux+Las- (could consider site directed mutagensis with Pallavi's primers) |
+ | Email Chris about genomic insert vector | ||
+ | Talk to Erin Feeney about the status of the LuxR and LasR cassettes | ||
</pre> | </pre> |
Revision as of 23:00, 10 September 2008
Wideloache 16:20, 10 September 2008 (EDT)
On Monday, I got K091136, K091146, and K091117 out of the -80. I need to make pLux' and pLux+Las- and do a genomic insertion of LuxR/LasR before I can test the XOR promoters.
Begin Primer Synthesis of pLux' and pLux+Las- (could consider site directed mutagensis with Pallavi's primers) Email Chris about genomic insert vector Talk to Erin Feeney about the status of the LuxR and LasR cassettes