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| − | == Las/Rhl cell signaling system ==
| + | '''[[Cellular Signaling Systems]]''' |
| − | '''Responsible''': Robert Cool, Alicia Allen, and Erin Feeney | |
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| − | '''Las System''' | + | '''[[XOR Gate Design]]''' |
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| − | '''Signal Molecule''': An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)
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| − | '''Bacterial species''': Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)
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| − | '''Receiver protein''': LasR
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| − | '''Effect of binding''': TXN activation of virulence genes, lasA, lasB, apr, toxR
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| − | '''Synthase''': LasI enzyme
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| − | '''Target Genes''': lasI, lasA, lasB, apr, toxR
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| − | '''Regulation''': unknown
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| − | '''References'''
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| − | "Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"
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| − | JP Pearson, EC Pesci and BH Iglewski
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| − | [http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=las&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]
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| − | "Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"
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| − | Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci
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| − | [http://jb.asm.org/cgi/content/full/187/13/4372?view=long&pmid=15968046 2]
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| − | Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa
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| − | Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden
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| − | [http://jb.asm.org/cgi/content/full/185/12/3558?view=long&pmid=12775693 3]
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| − | Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]
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| − | '''Rhl System'''
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| − | <br>
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| − | '''Signal Molecule''': An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)
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| − | '''Bacterial species''': Pseudomonas aeruginosa, gram(-)
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| − | '''Receiver Protein''': Rhl R
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| − | '''Effect of Binding''': activation of Rhamnosyl Transferase, then making RL (rhamnolipid)
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| − | '''Synthase''': RhlA and RhlB
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| − | '''Target Genes''': pqsABCDE and phnAB
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| − | '''Regulation''': unknown
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| − | '''References'''
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| − | [http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]
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| − | [[Image:Las_rhl.gif]] | |
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| − | '''Parts Needed''':
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| − | LasR + pro/term
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| − | RhlR + pro/term
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| − | RhlI + pro/term
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| − | pqsABCDE + pro/term
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| − | pqsR
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| − | pqsH + pro/term
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| − | phnAB
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| − | LasI
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| − | RBS
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| − | == Lux cell signaling system ==
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| − | '''Responsible:''' Andrew Gordon and Pallavi Penumetcha
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| − | '''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules
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| − | '''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli''
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| − | '''Receiver protein:''' LuxR protein receives signal from AHL; also has some control over transciption of luciferase
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| − | '''Signal molecule synthase:''' LuxI; also has some control over transciption of luciferase
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| − | '''Additional Information:''' "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
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| − | [[Image:800px-Luxrreceiverschematic.png]]
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| − | '''Resources'''
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| − | [http://partsregistry.org/Lux Lux Operon Pathway]
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| − | [http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]
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| − | [http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]
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| − | '''References'''
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| − | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]
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| − | [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation
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| − | '''Parts Needed''':
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| − | LuxR + pro/term
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| − | RBS
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| − | LuxI + pro/term
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| − | LuxI sender
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