Difference between revisions of "IGEM 2009 Project"
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| Leland Taylor || CCCUC || CUGAGGGUC-9 || Pro6 || '''1 clone 100% match''', putting pBAD upstream || 7 5mer+RFPs sequencing || 7 5mer+Tets sequencing | | Leland Taylor || CCCUC || CUGAGGGUC-9 || Pro6 || '''1 clone 100% match''', putting pBAD upstream || 7 5mer+RFPs sequencing || 7 5mer+Tets sequencing | ||
|- | |- | ||
| − | | Alyndria Thompson|| CGGUC || UUGACCGAC-9 || Arg2 || '''4 clones 100%match''', pBad is upstream clone || | + | | Alyndria Thompson|| CGGUC || UUGACCGAC-9 || Arg2 || '''4 clones 100%match''', pBad is upstream clone || '''3 clones matched''' to Nco1 - 5mer+RFPs sequencing || '''2 clones matche'''d to BamH1 - 5mer+Tets sequencing |
|- | |- | ||
| Clif Davis|| 5mer codon || Anticodon Sequence || Leu3|| tRNA Status || 5mer+RFP Status || 2 5mer+CAT sequenced-99% Match with a Conservative Mutation | | Clif Davis|| 5mer codon || Anticodon Sequence || Leu3|| tRNA Status || 5mer+RFP Status || 2 5mer+CAT sequenced-99% Match with a Conservative Mutation | ||
Revision as of 21:23, 17 July 2009
Biology Based:
- Which reporters are we going to use?
- What naming system are we going to use for the suppressor tRNAs?
- How do you build the tRNA construct?
- How are we going to build the 5-mer BioBricks?
Using Stop Codons to Truncate Translation
Davidson ATG+5mer BioBrick Sequences
Frameshift Mutation Leader PCR primers
Align E. coli tRNA sequences
Compare 2 tRNA structures
Team Progress Table
| Student Name | 5mer Codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+XFP Status | 5mer+Drug resistance Status |
|---|---|---|---|---|---|---|
| Olivia Ho-Shing | CCAUC | GUGAUCCAA-9 | Pro4 | 2 verified clones , both ligated with pBad | 2 5mer+RFPs sequencing | 5 5mer+Tets sequencing |
| Olivia Ho-Shing | CCAUC | UUUGAUGGAG-10 | Pro5 | 1 verified clone , both ligated with pBad | 2 5mer+RFPs sequencing | 5 5mer+Tets sequencing |
| Romina Clemente | CUAGU | UUACUAGAC-9 | Leu4 | 1 clone 100% match, putting pBAD upstream | 9 5mer+RFPs sequencing | 5 5mer+Tets sequencing |
| Shamita Punjabi | CCACU | CUAGUGGAC-9 | Pro3 | 2 clones being resequenced | 6 5mer+RFPs sequencing | 5 5mer+Tets sequencing |
| Leland Taylor | CCCUC | CUGAGGGUC-9 | Pro6 | 1 clone 100% match, putting pBAD upstream | 7 5mer+RFPs sequencing | 7 5mer+Tets sequencing |
| Alyndria Thompson | CGGUC | UUGACCGAC-9 | Arg2 | 4 clones 100%match, pBad is upstream clone | 3 clones matched to Nco1 - 5mer+RFPs sequencing | 2 clones matched to BamH1 - 5mer+Tets sequencing |
| Clif Davis | 5mer codon | Anticodon Sequence | Leu3 | tRNA Status | 5mer+RFP Status | 2 5mer+CAT sequenced-99% Match with a Conservative Mutation |
| Student Name | 5mer codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+RFP Status | 5mer+Tet Resistance Status |
| Student Name | 5mer codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+RFP Status | 5mer+Tet Resistance Status |
| Student Name | 5mer codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+RFP Status | 5mer+Tet Resistance Status |
| Student Name | 5mer codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+RFP Status | 5mer+Tet Resistance Status |
| Student Name | 5mer codon | Anticodon Sequence | Codon Short Name | tRNA Status | 5mer+RFP Status | 5mer+Tet Resistance Status |
Math Based: (Davidson) We have posted a Mathematica notebook on the Wiggio. Basically we are at a point where we can choose the number of variables, SAT number(2-SAT, 3-SAT, etc), and the number of clauses to use at a time and produce a table of inputs as the rows and clauses as the columns, with a 1 if the input satisfies the clause and a 0 if the input fails to satisfy the clause. In addition, we can produce a "Super Table," which lists all the SAT problems given the number of clauses in conjunctive normal form and displays how many of the clauses in a given problem each input satisfies. Feel free to view the posted Mathematica notebook; there are some instructions within the notebook itself. When you open it, there will be a window which asks about dynamic content; be sure to click "Enable Dynamic."
