Difference between revisions of "Olivia's Assignment"
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I want to explore the question '''Does high salinity affect the proton pump?''' So I'd like to compare the rhodopsin hydrogen pump gene(s) found in H. mukohataei to those found in other halophiles and non-halophiles. | I want to explore the question '''Does high salinity affect the proton pump?''' So I'd like to compare the rhodopsin hydrogen pump gene(s) found in H. mukohataei to those found in other halophiles and non-halophiles. | ||
| + | Goals/To Do List: | ||
| + | *Find opsin gene(s) for H. mukohataei and other related halophiles | ||
| + | *Find opsin genes in other archaea | ||
| + | *BLASTp and BLASTn | ||
| + | *Compare GC content - does it look like the rest of the host genome | ||
| + | *Make phylogenetic tree based on opsin | ||
Using JGI, I found these known protein sequences predicted to be the proton pump in H. mukohataei: | Using JGI, I found these known protein sequences predicted to be the proton pump in H. mukohataei: | ||
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*[http://img.jgi.doe.gov/cgi-bin/geba/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=644032598 644032598] bacteriorhodopsin<br> | *[http://img.jgi.doe.gov/cgi-bin/geba/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=644032598 644032598] bacteriorhodopsin<br> | ||
[[Image:Mukohataei-rhodopsin.png|thumb|center|500px]]<br> | [[Image:Mukohataei-rhodopsin.png|thumb|center|500px]]<br> | ||
| + | |||
| + | [[http://temp.geobio.uib.no/uploads/documents/species_in_haloarchaea.pdf]] | ||
| + | |||
| + | == Other Species found to have Bacteriorhodopsin == | ||
| + | *Natronomonas pharaonis | ||
| + | *H. vallismortis (ATCC 29715) sopII and htrII genes for sensory rhodopsin II apoprotein and sensory rhodopsin II transducer ACCESSION Z35308 | ||
| + | * | ||
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| + | |||
| + | I searched the [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=110065 Conserved Domains Database for pFam01036] bacteriorhodopsin. | ||
Revision as of 16:15, 22 September 2009
I want to explore the question Does high salinity affect the proton pump? So I'd like to compare the rhodopsin hydrogen pump gene(s) found in H. mukohataei to those found in other halophiles and non-halophiles. Goals/To Do List:
- Find opsin gene(s) for H. mukohataei and other related halophiles
- Find opsin genes in other archaea
- BLASTp and BLASTn
- Compare GC content - does it look like the rest of the host genome
- Make phylogenetic tree based on opsin
Using JGI, I found these known protein sequences predicted to be the proton pump in H. mukohataei:
- 644030941 bacteriorhodopsin
- 644032376 bacteriorhodopsin
- 644032569 Bacteriorhodopsin
- 644032598 bacteriorhodopsin
[[1]]
Other Species found to have Bacteriorhodopsin
- Natronomonas pharaonis
- H. vallismortis (ATCC 29715) sopII and htrII genes for sensory rhodopsin II apoprotein and sensory rhodopsin II transducer ACCESSION Z35308
I searched the Conserved Domains Database for pFam01036 bacteriorhodopsin.
