Difference between revisions of "LuxR/cI"
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| + | '''Parallel Biobrick Assembly''' | ||
| + | |||
'''Objective 1''': pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance) | '''Objective 1''': pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance) | ||
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| 1B || B0034 || RBS || RX/SP || RX || 12bp || K091109 || LuxS Gene || RX/SP || RS || 516bp || || | | 1B || B0034 || RBS || RX/SP || RX || 12bp || K091109 || LuxS Gene || RX/SP || RS || 516bp || || | ||
|- | |- | ||
| − | | || || || || | + | | 2 || B0034 || RBS || RX/SP || SP || 12bp || Product of 1B || LuxS + RBS || RX/SP || XP || || || |
| + | |- | ||
| + | | 3 || Product of 2 || RBS + LuxS + RBS || RX/SP || SP || || Product of 1A || Mnt Gene + TT || RX/SP || XP || || || | ||
| + | |- | ||
| + | | 4 || K091107 || pLux/cI || RX/SP || SP || || Product of 3 || RBS + LuxS + RBS + Mnt + TT || RX/SP || XP || || Objective 1 || | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | |||
| + | '''Parts''' | ||
| + | {| border="1" cellpadding="2" | ||
| + | !width="40"|BBa_ | ||
| + | !width="110"|Description | ||
| + | !width="60"|Source | ||
| + | !width="100"|location('07) | ||
| + | !width="35"|Vector | ||
|- | |- | ||
| − | | || || | + | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 |
|- | |- | ||
| − | | || || | + | |[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 |
|- | |- | ||
| − | | || || | + | |[http://partsregistry.org/Part:BBa_C0072 C0072] ||Mnt Gene ||registry 07 ||Plt.2-5M ||pSB2K3 |
|- | |- | ||
| − | | || || | + | |[http://partsregistry.org/Part:BBa_K091107 K091107] ||pLux/cI || N/A || || |
|- | |- | ||
| − | | || || | + | |[http://partsregistry.org/Part:BBa_K091109 K091109] ||LuxS || N/A || || |
|- | |- | ||
|} | |} | ||
Latest revision as of 16:17, 2 July 2008
Parallel Biobrick Assembly
Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)
K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1A | B0015 | Double Terminator | RX/SP | RX | 129bp | C0072 | Mnt Gene | RX/SP | RS | 288bp | ||
| 1B | B0034 | RBS | RX/SP | RX | 12bp | K091109 | LuxS Gene | RX/SP | RS | 516bp | ||
| 2 | B0034 | RBS | RX/SP | SP | 12bp | Product of 1B | LuxS + RBS | RX/SP | XP | |||
| 3 | Product of 2 | RBS + LuxS + RBS | RX/SP | SP | Product of 1A | Mnt Gene + TT | RX/SP | XP | ||||
| 4 | K091107 | pLux/cI | RX/SP | SP | Product of 3 | RBS + LuxS + RBS + Mnt + TT | RX/SP | XP | Objective 1 |
Parts
| BBa_ | Description | Source | location('07) | Vector |
|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 |
| C0072 | Mnt Gene | registry 07 | Plt.2-5M | pSB2K3 |
| K091107 | pLux/cI | N/A | ||
| K091109 | LuxS | N/A |
Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1 | ||||||||||||
