Difference between revisions of "Divide and Conquer Biological Challenges"
From GcatWiki
MaCampbell (talk | contribs) (→* We need to get the Lsr/AI-2 system working.) |
MaCampbell (talk | contribs) (→* We need to get the Lsr/AI-2 system working.) |
||
| Line 6: | Line 6: | ||
* There are '''NO''' Lsr parts in the registry: <br> | * There are '''NO''' Lsr parts in the registry: <br> | ||
*We will need to make BB parts for:<br> | *We will need to make BB parts for:<br> | ||
| − | # LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp. | + | # LsrR and LsrK which are adjacent to each other in the E. coli genome. [http://www.ncbi.nlm.nih.gov/projects/sviewer/?id=NC_010473.1&v=1687890..1688937 We could amplify them together with a total size of over 2600bp.] |
# Lsr promoter | # Lsr promoter | ||
# LuxS that produces DPD that somehow is converted to ''R''-THMF. | # LuxS that produces DPD that somehow is converted to ''R''-THMF. | ||
Revision as of 21:06, 28 May 2008
Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT: (insert links to examples here)
- AND: (insert links to examples here)
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
