Difference between revisions of "Divide and Conquer Biological Challenges"
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MaCampbell (talk | contribs) (→* We need to get the Lsr/AI-2 system working.) |
(→* We need to have an XOR logic gate produced.) |
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=== * We need to have an XOR logic gate produced. === | === * We need to have an XOR logic gate produced. === | ||
'''Existing Logic Gates''' | '''Existing Logic Gates''' | ||
| − | *NOT: ( | + | *NOT:[http://partsregistry.org/Part:BBa_I732205 I732205 (NOT/ Dual-repressed NOR)] |
*AND: (insert links to examples here) | *AND: (insert links to examples here) | ||
| + | *NAND:[http://partsregistry.org/Part:BBa_I732914 I732914] | ||
| + | *NOR:[http://partsregistry.org/Part:BBa_I732916 I732916], [http://partsregistry.org/Part:BBa_I732917 I732917] | ||
| + | *Inverters:[http://partsregistry.org/Part:BBa_J23040 J23040 (AHL-dependent inverter)], | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04121 Q04121 (lac inverter)], | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04400 Q04400 (tet inverter)] | ||
| + | |||
=== * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon. === | === * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon. === | ||
What if Amp<sup>R</sup> is secreted and cells are not Amp<sup>R</sup>? This would prevent cells down the chain from responding too soon. | What if Amp<sup>R</sup> is secreted and cells are not Amp<sup>R</sup>? This would prevent cells down the chain from responding too soon. | ||
Revision as of 21:09, 28 May 2008
Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT:I732205 (NOT/ Dual-repressed NOR)
- AND: (insert links to examples here)
- NAND:I732914
- NOR:I732916, I732917
- Inverters:J23040 (AHL-dependent inverter),
Q04121 (lac inverter), Q04400 (tet inverter)
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
