Difference between revisions of "Davidson Missouri W/CUGI Sequencing"
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*Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed. | *Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed. | ||
*$3.00 / sample | *$3.00 / sample | ||
| + | *VR and VF2 melting temperatures are 60 C. | ||
Note by ATM: | Note by ATM: | ||
I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions. | I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions. | ||
Revision as of 17:45, 3 March 2009
For Dr. Campbell's Lab
The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The user name is Dr. C's email prefix. The website is www.genome.clemson.edu
- You need to send at least 30 ng of DNA in a tube for each sequencing reaction. Unless you have a good reason not to, always submit 90 ng for each primer used for sequencing.
- The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume.
- The DNA to be sequenced must be sent dried down, or within 3 µl. Unless you have a good reason not to, always submit the DNA dried down.
- Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed.
- $3.00 / sample
- VR and VF2 melting temperatures are 60 C.
Note by ATM: I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions.
