Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"
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| − | RBS | + | RBS+lamba cI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K082022 K082022] |
| − | RBS | + | pBAD+RBS+LacI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_S03980 S03980] |
| − | RBS+tetR [http://partsregistry.org/wiki/index.php/Part: | + | pBAD+RBS+tetR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091145 K091145] |
| − | RBS | + | pBAD+RBS+luxR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091179 K091179] |
RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500] | RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500] | ||
Revision as of 16:45, 20 May 2009
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
Experimental Design
Parts Needed
Promoters
pR R0051
pLacIQ1 K091112
pTetR R0040
pLuxR R0062
Genes
RBS+lamba cI+TT K082022
pBAD+RBS+LacI+TT S03980
pBAD+RBS+tetR+TT K091145
pBAD+RBS+luxR+TT K091179
RBS(B0030)+GFP E5500
RBS(B0030)+RFP S04120
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]
luxO luxT [2]
PprpB propionate [3]
