Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"

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(Parts Needed)
(Parts Needed)
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== Parts Needed ==
 
== Parts Needed ==
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'''Parts from Registry'''
  
 
'''Promoters'''  
 
'''Promoters'''  
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Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]
 
Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]
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'''Parts needed to be built from scratch'''
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Pprp
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prpB
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pLuxT
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frame shift suppressor 1
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frame shift suppressor 2
  
 
== References ==
 
== References ==

Revision as of 19:12, 20 May 2009

Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,

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Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

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Experimental Design

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Parts Needed

Parts from Registry

Promoters

pR R0051

pLacIQ1 K091112

pTetR R0040

pLuxR R0062

pMnt R0073

Genes

RBS+lamba cI+TT K082022

pBAD+RBS+LacI+TT S03980

pBAD+RBS+tetR+TT K091145

pBAD+RBS+luxR+TT K091179

RBS(B0030)+GFP E5500

RBS(B0030)+RFP S04120

Mnt C0072

Parts needed to be built from scratch

Pprp

prpB

pLuxT

frame shift suppressor 1

frame shift suppressor 2

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]

luxO luxT [2]

PprpB propionate [3]