Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"
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| − | pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051] | + | pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051]2009 Kit Plate 1 6K pSB1A2 |
| − | pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112] | + | pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112]DC |
| − | pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040] | + | pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040]2009 Kit Plate 1 6I pSB1A2 |
| − | pLuxR [http://partsregistry.org/wiki/index.php/Part:BBa_R0062 R0062] | + | pLuxR [http://partsregistry.org/wiki/index.php/Part:BBa_R0062 R0062]2009 Kit Plate 1 6O pSB1A2 |
| − | pMnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073] | + | pMnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073]2009 Kit Plate 1 16B pSB2K3 |
'''Genes''' | '''Genes''' | ||
| − | RBS+lamba cI+TT [http://partsregistry.org/wiki/index.php/Part: | + | RBS+lamba cI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_P0451 P0451]2009 Kit Plate 2 1N pSB1AK3 |
| − | pBAD+RBS+LacI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_S03980 S03980] | + | pBAD+RBS+LacI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_S03980 S03980]MWSU |
| − | pBAD+RBS+tetR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091145 K091145] | + | pBAD+RBS+tetR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091145 K091145]MWSU |
| − | pBAD+RBS+luxR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091179 K091179] | + | pBAD+RBS+luxR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091179 K091179]MWSU |
| − | RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500] | + | RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500]2007 Parts Kit Plate 1 9J pSB1A2 |
| − | RBS(B0030)+RFP [http://partsregistry.org/wiki/index.php/Part:BBa_S04120 S04120] | + | RBS(B0030)+RFP [http://partsregistry.org/wiki/index.php/Part:BBa_S04120 S04120]upon request |
| − | Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072] | + | Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]2009 Kit Plate 1 12L pSB2K3 |
| − | LuxO-P [http://partsregistry.org/wiki/index.php?title=Part:BBa_K131022 K131022] | + | LuxO-P [http://partsregistry.org/wiki/index.php?title=Part:BBa_K131022 K131022]2009 Kit Plate 3 8K pSB1AC3 |
'''Parts needed to be built from scratch''' | '''Parts needed to be built from scratch''' | ||
Revision as of 15:55, 21 May 2009
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
Experimental Design
Parts Needed
Parts from Registry
Promoters
pR R00512009 Kit Plate 1 6K pSB1A2
pLacIQ1 K091112DC
pTetR R00402009 Kit Plate 1 6I pSB1A2
pLuxR R00622009 Kit Plate 1 6O pSB1A2
pMnt R00732009 Kit Plate 1 16B pSB2K3
Genes
RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3
pBAD+RBS+LacI+TT S03980MWSU
pBAD+RBS+tetR+TT K091145MWSU
pBAD+RBS+luxR+TT K091179MWSU
RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2
RBS(B0030)+RFP S04120upon request
Mnt C00722009 Kit Plate 1 12L pSB2K3
LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3
Parts needed to be built from scratch
Pprp sequence within the prp operon[1]
prpB 2-methylisocitrate lyase in E.coli MG1655 [2]
pLuxT
frame shift suppressor 1
frame shift suppressor 2
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]
luxO luxT [4]
PprpB propionate [5]
