Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"

From GcatWiki
Jump to: navigation, search
(Parts Needed)
(Parts Needed)
Line 34: Line 34:
 
'''Promoters'''  
 
'''Promoters'''  
  
pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051]
+
pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051]2009 Kit Plate 1 6K pSB1A2
  
pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112]
+
pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112]DC
  
pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040]
+
pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040]2009 Kit Plate 1 6I pSB1A2
  
pLuxR [http://partsregistry.org/wiki/index.php/Part:BBa_R0062 R0062]
+
pLuxR [http://partsregistry.org/wiki/index.php/Part:BBa_R0062 R0062]2009 Kit Plate 1 6O pSB1A2
  
pMnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073]
+
pMnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073]2009 Kit Plate 1 16B pSB2K3
  
 
'''Genes'''
 
'''Genes'''
  
RBS+lamba cI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K082022 K082022]
+
RBS+lamba cI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_P0451 P0451]2009 Kit Plate 2 1N pSB1AK3
  
pBAD+RBS+LacI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_S03980 S03980]
+
pBAD+RBS+LacI+TT [http://partsregistry.org/wiki/index.php/Part:BBa_S03980 S03980]MWSU
  
pBAD+RBS+tetR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091145 K091145]
+
pBAD+RBS+tetR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091145 K091145]MWSU
  
pBAD+RBS+luxR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091179 K091179]
+
pBAD+RBS+luxR+TT [http://partsregistry.org/wiki/index.php/Part:BBa_K091179 K091179]MWSU
  
RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500]
+
RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500]2007 Parts Kit Plate 1 9J pSB1A2
  
RBS(B0030)+RFP [http://partsregistry.org/wiki/index.php/Part:BBa_S04120 S04120]
+
RBS(B0030)+RFP [http://partsregistry.org/wiki/index.php/Part:BBa_S04120 S04120]upon request
  
Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]
+
Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]2009 Kit Plate 1 12L pSB2K3
  
LuxO-P [http://partsregistry.org/wiki/index.php?title=Part:BBa_K131022 K131022]
+
LuxO-P [http://partsregistry.org/wiki/index.php?title=Part:BBa_K131022 K131022]2009 Kit Plate 3 8K pSB1AC3
  
 
'''Parts needed to be built from scratch'''
 
'''Parts needed to be built from scratch'''

Revision as of 15:55, 21 May 2009

Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Experimental Design

Error creating thumbnail: Unable to save thumbnail to destination

Error creating thumbnail: Unable to save thumbnail to destination

Parts Needed

Parts from Registry

Promoters

pR R00512009 Kit Plate 1 6K pSB1A2

pLacIQ1 K091112DC

pTetR R00402009 Kit Plate 1 6I pSB1A2

pLuxR R00622009 Kit Plate 1 6O pSB1A2

pMnt R00732009 Kit Plate 1 16B pSB2K3

Genes

RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3

pBAD+RBS+LacI+TT S03980MWSU

pBAD+RBS+tetR+TT K091145MWSU

pBAD+RBS+luxR+TT K091179MWSU

RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2

RBS(B0030)+RFP S04120upon request

Mnt C00722009 Kit Plate 1 12L pSB2K3

LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3

Parts needed to be built from scratch

Pprp sequence within the prp operon[1]

prpB 2-methylisocitrate lyase in E.coli MG1655 [2]

pLuxT

frame shift suppressor 1

frame shift suppressor 2

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]

luxO luxT [4]

PprpB propionate [5]