Difference between revisions of "Blasting Spacers for Our Genome"

Revision as of 17:47, 5 November 2009

Blasting Spacers

CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.

Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

This image is only part of it. . .

NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC

No significant hits. . . lowest E-value is 1.6

Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA

Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC

Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG

No significant hits. . . lowest E-value is 1.6

Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC

No significant hits . . . lowest E-value is 1.5

Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT

No significant hits . . . lowest E-value is 1.4

Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC

No significant hits . . . lowest E-value is 6.4

Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT