Difference between revisions of "Blasting Spacers for Our Genome"
Karicheson (talk | contribs) (→Blasting Spacers) |
Karicheson (talk | contribs) (→Blasting Spacers) |
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| Line 8: | Line 8: | ||
Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG | Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG | ||
| − | |||
| − | |||
Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA | Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA | ||
| − | |||
Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA | Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA | ||
| − | + | ||
Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG | Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG | ||
| − | + | ||
Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC | Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG | Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG | ||
| − | |||
| − | |||
Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG | Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG | ||
| − | + | ||
Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC | Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC | ||
| − | |||
| − | + | ||
Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC | Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC | ||
| − | |||
Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA | Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA | ||
| − | + | ||
Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC | Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC | ||
| − | + | ||
Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC | Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC | ||
| − | |||
Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA | Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA | ||
| − | |||
Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC | Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC | ||
| − | + | ||
Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG | Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG | ||
| − | + | ||
Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC | Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC | ||
| − | + | ||
Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT | Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT | ||
| − | + | ||
Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC | Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC | ||
| − | + | ||
Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT | Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT | ||
| − | + | ||
Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT | Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT | ||
| − | + | ||
Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG | Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG | ||
| − | + | ||
Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA | Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA | ||
| − | + | ||
Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG | Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG | ||
| − | + | ||
Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG | Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG | ||
| − | + | ||
Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT | Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT | ||
| − | + | ||
Spacer Twenty-Six: TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG | Spacer Twenty-Six: TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG | ||
| − | + | ||
Spacer Twenty 27: TCCTCCTTGTACCCACGGTCTTGCCGATCCATCCCG | Spacer Twenty 27: TCCTCCTTGTACCCACGGTCTTGCCGATCCATCCCG | ||
| − | + | ||
Spacer Twenty-Eight: GACTGGCGTGTTGCCGTTCAGGCCGGCGTTGATCCCG | Spacer Twenty-Eight: GACTGGCGTGTTGCCGTTCAGGCCGGCGTTGATCCCG | ||
| − | + | ||
Spacer Twenty-Nine: CTCAGCAGCAGTCAACGGCATTTTATACACCTTGT | Spacer Twenty-Nine: CTCAGCAGCAGTCAACGGCATTTTATACACCTTGT | ||
| − | + | ||
Spacer Thirty: CACCCCTTCCGGGGAGACGAGGAAACCCCGGACGA | Spacer Thirty: CACCCCTTCCGGGGAGACGAGGAAACCCCGGACGA | ||
| − | |||
Spacer Thirty-One: GTCACGCTGTCTGACGATATGGCTGACCAGGTGC | Spacer Thirty-One: GTCACGCTGTCTGACGATATGGCTGACCAGGTGC | ||
| − | + | ||
Spacer Thirty-Two: CACTCCTGGGCGGCCTCATCGGCGGCCATCGTC | Spacer Thirty-Two: CACTCCTGGGCGGCCTCATCGGCGGCCATCGTC | ||
| − | + | ||
Spacer Thirty-Three:GTTGTGTGAGGTATGCGATGGACACCACCGATCACG | Spacer Thirty-Three:GTTGTGTGAGGTATGCGATGGACACCACCGATCACG | ||
| − | + | ||
| Line 158: | Line 141: | ||
[[Image:crispr3.jpg]] | [[Image:crispr3.jpg]] | ||
| − | Spacer One: TCCGAGACGTGTTCCCTCTCTAGCTGTGCATCTTCC | + | '''Spacer One:''' TCCGAGACGTGTTCCCTCTCTAGCTGTGCATCTTCC |
LOTS of Hits!!!! | LOTS of Hits!!!! | ||
| Line 166: | Line 149: | ||
No significant hits . . . lowest E-value is 1.5 | No significant hits . . . lowest E-value is 1.5 | ||
| − | Spacer Three: GATCCGGAATATGAAGTGACGAACGATCCGGATACGG | + | '''Spacer Three:''' GATCCGGAATATGAAGTGACGAACGATCCGGATACGG |
[[Image:crispr2spacer3.jpg]] | [[Image:crispr2spacer3.jpg]] | ||
| − | Spacer Four: TCGACGAGATCGGCGCGAACTCGTTCGCTGATACT | + | '''Spacer Four''': TCGACGAGATCGGCGCGAACTCGTTCGCTGATACT |
[[Image:crispr2spacer4.jpg]] | [[Image:crispr2spacer4.jpg]] | ||
| − | Spacer Five: TCGGGGACCGAGACGACGGGGCCGGGTGCTGTCT | + | '''Spacer Five''': TCGGGGACCGAGACGACGGGGCCGGGTGCTGTCT |
[[Image:crispr2spacer5.jpg]] | [[Image:crispr2spacer5.jpg]] | ||
| − | Spacer Six: CCGGAGGGGCCGCTGCGTGGGTGATCTGGAGAGAAGA | + | '''Spacer Six''': CCGGAGGGGCCGCTGCGTGGGTGATCTGGAGAGAAGA |
[[Image:crispr2spacer6.jpg]] | [[Image:crispr2spacer6.jpg]] | ||
| Line 186: | Line 169: | ||
No significant hits . . . lowest E-value is 5.5 | No significant hits . . . lowest E-value is 5.5 | ||
| − | Spacer Eight: ACGGAAATCCAGCCGATCACCCTCCGAGAGGAGAGG | + | '''Spacer Eight:''' ACGGAAATCCAGCCGATCACCCTCCGAGAGGAGAGG |
[[Image:crispr2spacer8.jpg]] | [[Image:crispr2spacer8.jpg]] | ||
| Line 194: | Line 177: | ||
No significant hits . . . lowest E-value is 5.2 | No significant hits . . . lowest E-value is 5.2 | ||
| − | Spacer Ten: CGGGAAGACCACGACCGCCCGCGCCCTCCAGTTCGA | + | '''Spacer Ten''': CGGGAAGACCACGACCGCCCGCGCCCTCCAGTTCGA |
[[Image:crispr2spacer10.jpg]] | [[Image:crispr2spacer10.jpg]] | ||
| − | Spacer Eleven: GGCTTCTACGTCGGCAACCGGACCGAGGACGGCGATG | + | '''Spacer Eleven''': GGCTTCTACGTCGGCAACCGGACCGAGGACGGCGATG |
[[Image:crispr2spacer11.jpg]] | [[Image:crispr2spacer11.jpg]] | ||
| − | Spacer Twelve: AAGTACGCCTCGATCATCAACGGCGTCCGGGCTGT | + | '''Spacer Twelve''': AAGTACGCCTCGATCATCAACGGCGTCCGGGCTGT |
[[Image:crispr2spacer12.jpg]] | [[Image:crispr2spacer12.jpg]] | ||
| Line 218: | Line 201: | ||
No significant hits . . . lowest E-value is 1.4 | No significant hits . . . lowest E-value is 1.4 | ||
| − | Spacer Sixteen: TCGTCGAGCGGCAGGCCGCCGACCGCTACGGGCTG | + | '''Spacer Sixteen''': TCGTCGAGCGGCAGGCCGCCGACCGCTACGGGCTG |
[[Image:crispr2spacer16.jpg]] | [[Image:crispr2spacer16.jpg]] | ||
| − | Spacer Seventeen: CGACACGGTCGAAGATGCTGGCAGCTCGCGAATCGC | + | '''Spacer Seventeen:''' CGACACGGTCGAAGATGCTGGCAGCTCGCGAATCGC |
[[Image:crispr2spacer17.jpg]] | [[Image:crispr2spacer17.jpg]] | ||
| − | Spacer Eighteen: GGATCTCGACGGCCGCGTGGCCGTGCATCTCGGGGTC | + | '''Spacer Eighteen:''' GGATCTCGACGGCCGCGTGGCCGTGCATCTCGGGGTC |
[[Image:crispr2spacer18.jpg]] | [[Image:crispr2spacer18.jpg]] | ||
| Line 242: | Line 225: | ||
No significant hits . . . lowest E-value is 1.5 | No significant hits . . . lowest E-value is 1.5 | ||
| − | Spacer Twenty-Two: CGATACGTCCGGGACGCCCGTGACGACCACCACTGC | + | '''Spacer Twenty-Two''': CGATACGTCCGGGACGCCCGTGACGACCACCACTGC |
[[Image:crispr2spacer21.jpg]] | [[Image:crispr2spacer21.jpg]] | ||
| Line 250: | Line 233: | ||
No significant hits . . . lowest E-value is 1.4 | No significant hits . . . lowest E-value is 1.4 | ||
| − | Spacer Twenty-Four: GAGCATCTCTCCAATCAGCGGTCATCAACCGCGA | + | '''Spacer Twenty-Four''': GAGCATCTCTCCAATCAGCGGTCATCAACCGCGA |
[[Image:crispr2spacer24.jpg]] | [[Image:crispr2spacer24.jpg]] | ||
| Line 270: | Line 253: | ||
No significant hits . . . lowest E-value is 5.2 | No significant hits . . . lowest E-value is 5.2 | ||
| − | Spacer Twenty-Nine: CTGGCCTACCCGAGTCGGTTCCCCGCAGCACTCGCA | + | '''Spacer Twenty-Nine:''' CTGGCCTACCCGAGTCGGTTCCCCGCAGCACTCGCA |
[[Image:crispr2spacer29.jpg]] | [[Image:crispr2spacer29.jpg]] | ||
| − | Spacer Thirty: ACGATCGTCACCGACACCCTCGGGGCCGGCGGCGC | + | '''Spacer Thirty''': ACGATCGTCACCGACACCCTCGGGGCCGGCGGCGC |
[[Image:crispr2spacer30.jpg]] | [[Image:crispr2spacer30.jpg]] | ||
| Line 282: | Line 265: | ||
No significant hits . . . lowest E-value is 5.6 | No significant hits . . . lowest E-value is 5.6 | ||
| − | Spacer Thirty-Two: TTAGATGATCAGGTAGCCTGCTACCAGTGCAGCTGC | + | '''Spacer Thirty-Two:''' TTAGATGATCAGGTAGCCTGCTACCAGTGCAGCTGC |
[[Image:crispr2spacer32.jpg]] | [[Image:crispr2spacer32.jpg]] | ||
| − | Spacer Thirty-Three: GAGACCCAGCTTTGCCTTCCAGGTGATCAGCTCGTA | + | '''Spacer Thirty-Three:''' GAGACCCAGCTTTGCCTTCCAGGTGATCAGCTCGTA |
[[Image:crispr2spacer33.jpg]] | [[Image:crispr2spacer33.jpg]] | ||
| − | Spacer Thirty-Four: TCGAAGCGCTCGGTCGCGACGGAGACCAGCGACCAGCTG | + | '''Spacer Thirty-Four:''' TCGAAGCGCTCGGTCGCGACGGAGACCAGCGACCAGCTG |
[[Image:crispr2spacer34.jpg]] | [[Image:crispr2spacer34.jpg]] | ||
| − | Spacer Thirty-Five: TGACGACCACACACACGAGGCCGTGCGTGTGCTTGTA | + | '''Spacer Thirty-Five:''' TGACGACCACACACACGAGGCCGTGCGTGTGCTTGTA |
[[Image:crispr2spacer35.jpg]] | [[Image:crispr2spacer35.jpg]] | ||
| − | Spacer Thirty-Six: CCACGTCCCGGTGACACGCAGCTCGGTGAGATCGC | + | '''Spacer Thirty-Six:''' CCACGTCCCGGTGACACGCAGCTCGGTGAGATCGC |
[[Image:crispr2spacer36.jpg]] | [[Image:crispr2spacer36.jpg]] | ||
| Line 306: | Line 289: | ||
No significant hits . . . lowest E-value is 5.9 | No significant hits . . . lowest E-value is 5.9 | ||
| − | Spacer Thirty-Eight: CGCAACCCGACGATCGAGGACGGGCCGTCCCTGGA | + | '''Spacer Thirty-Eight:''' CGCAACCCGACGATCGAGGACGGGCCGTCCCTGGA |
[[Image:crispr2spacer38.jpg]] | [[Image:crispr2spacer38.jpg]] | ||
| Line 318: | Line 301: | ||
No significant hits . . . lowest E-value is 5.2 | No significant hits . . . lowest E-value is 5.2 | ||
| − | Spacer Forty-One: TACGCTCGACGACCTCCGTCGTGCGCTCCAGAAGTCA | + | '''Spacer Forty-One:''' TACGCTCGACGACCTCCGTCGTGCGCTCCAGAAGTCA |
[[Image:crispr2spacer41.jpg]] | [[Image:crispr2spacer41.jpg]] | ||
Revision as of 19:55, 11 November 2009
Blasting Spacers
CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.
Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG
Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA
Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA
Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG
Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC
Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG
Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG
Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC
Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC
Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA
Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC
Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC
Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA
Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC
Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG
Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC
Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT
Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC
Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT
Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT
Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG
Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA
Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG
Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG
Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT
Spacer Twenty-Six: TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG
Spacer Twenty 27: TCCTCCTTGTACCCACGGTCTTGCCGATCCATCCCG
Spacer Twenty-Eight: GACTGGCGTGTTGCCGTTCAGGCCGGCGTTGATCCCG
Spacer Twenty-Nine: CTCAGCAGCAGTCAACGGCATTTTATACACCTTGT
Spacer Thirty: CACCCCTTCCGGGGAGACGAGGAAACCCCGGACGA
Spacer Thirty-One: GTCACGCTGTCTGACGATATGGCTGACCAGGTGC
Spacer Thirty-Two: CACTCCTGGGCGGCCTCATCGGCGGCCATCGTC
Spacer Thirty-Three:GTTGTGTGAGGTATGCGATGGACACCACCGATCACG
CRISPR Two Spacers: CHANGED parameters to be 1.-1 match/mistatch to make it less intense. . . also excluded eukaryotes NOW
Spacer One: TCCGAGACGTGTTCCCTCTCTAGCTGTGCATCTTCC
LOTS of Hits!!!!
Spacer Two: CAGATCTAAAACAATGTCATACGGAAAAATCGACATC
No significant hits . . . lowest E-value is 1.5
Spacer Three: GATCCGGAATATGAAGTGACGAACGATCCGGATACGG
Spacer Four: TCGACGAGATCGGCGCGAACTCGTTCGCTGATACT
Spacer Five: TCGGGGACCGAGACGACGGGGCCGGGTGCTGTCT
Spacer Six: CCGGAGGGGCCGCTGCGTGGGTGATCTGGAGAGAAGA
Spacer Seven: GTTGCGTGAGCTAGCGAAACACCGAGTCCGTGTGAT
No significant hits . . . lowest E-value is 5.5
Spacer Eight: ACGGAAATCCAGCCGATCACCCTCCGAGAGGAGAGG
Spacer Nine: CGGACATTCAGAAGCGCCTGACTAACCGCATGGCT
No significant hits . . . lowest E-value is 5.2
Spacer Ten: CGGGAAGACCACGACCGCCCGCGCCCTCCAGTTCGA
Spacer Eleven: GGCTTCTACGTCGGCAACCGGACCGAGGACGGCGATG
Spacer Twelve: AAGTACGCCTCGATCATCAACGGCGTCCGGGCTGT
Spacer Thirteen: GATGCTGTTGAGCTCGTAGCGATCCCACTCGGCGT
No significant hits . . . lowest E-value is 1.3
Spacer Fourteen: AGCCCTTGTGCAATGATCGGGAGTGCAATCCGACC
No significant hits . . . lowest E-value is 5.2
Spacer Fifteen: TCAGGCGAGTTGTCGGACGAACAGCTTGAAGCGTGT
No significant hits . . . lowest E-value is 1.4
Spacer Sixteen: TCGTCGAGCGGCAGGCCGCCGACCGCTACGGGCTG
Spacer Seventeen: CGACACGGTCGAAGATGCTGGCAGCTCGCGAATCGC
Spacer Eighteen: GGATCTCGACGGCCGCGTGGCCGTGCATCTCGGGGTC
Spacer Nineteen: AACGCTTCAACGCGCTCTATTGACCGAGCGTATCG
No significant hits . . . lowest E-value is 1.3
Spacer Twenty: AGATCTCGCGGATAAGCTGCCCCCGCCCTCCCATGAG
No significant hits . . . lowest E-value is 5.9
Spacer Twenty-One: CACTTCGAGCGGACTTTGGGCCACCCCGGAAAGTCAG
No significant hits . . . lowest E-value is 1.5
Spacer Twenty-Two: CGATACGTCCGGGACGCCCGTGACGACCACCACTGC
Spacer Twenty-Three: GGAGGAGCGGATGGACATGAGCGACACGACGATCCG
No significant hits . . . lowest E-value is 1.4
Spacer Twenty-Four: GAGCATCTCTCCAATCAGCGGTCATCAACCGCGA
Spacer Twenty-Five: AGTATCTGTCTACGCGATACCGCACCGTCAGAGTG
No significant hits . . . lowest E-value is 1.3
Spacer Twenty-Six: TACGACCTTCCCACTGAGGGTCTTGAGCTAACGATT
No significant hits . . . lowest E-value is 5.6
Spacer Twenty-Seven: GCGACGTGCTTACCTCTGACGCCAATATTGACCTT
No significant hits . . . lowest E-value is 5.2
Spacer Twenty-Eight: TTCATCAAGAGGCACACAAGCATGGTGCGTCCAAA
No significant hits . . . lowest E-value is 5.2
Spacer Twenty-Nine: CTGGCCTACCCGAGTCGGTTCCCCGCAGCACTCGCA
Spacer Thirty: ACGATCGTCACCGACACCCTCGGGGCCGGCGGCGC
Spacer Thirty-One: GAAACGACCGAGACGCAAAGCGAGTTCACACAACTC
No significant hits . . . lowest E-value is 5.6
Spacer Thirty-Two: TTAGATGATCAGGTAGCCTGCTACCAGTGCAGCTGC
Spacer Thirty-Three: GAGACCCAGCTTTGCCTTCCAGGTGATCAGCTCGTA
Spacer Thirty-Four: TCGAAGCGCTCGGTCGCGACGGAGACCAGCGACCAGCTG
Spacer Thirty-Five: TGACGACCACACACACGAGGCCGTGCGTGTGCTTGTA
Spacer Thirty-Six: CCACGTCCCGGTGACACGCAGCTCGGTGAGATCGC
Spacer Thirty-Seven: CATGGAGTCTTCAACATTTCATGGGCTGGGCTTGGCC
No significant hits . . . lowest E-value is 5.9
Spacer Thirty-Eight: CGCAACCCGACGATCGAGGACGGGCCGTCCCTGGA
Spacer Thirty-Nine: GCGTCGGACTGCGTCGATAGTGTTCGTGCTCATGTT
No significant hits . . . lowest E-value is 1.4
Spacer Forty: CAGACTTCTACTGGAAGGCGAAAACTGAGAAGGCA
No significant hits . . . lowest E-value is 5.2
Spacer Forty-One: TACGCTCGACGACCTCCGTCGTGCGCTCCAGAAGTCA
Spacer Forty-Two: GCGCTGCGGACGTGGTGTCAGAGGGGTTACCAGTAACT
No significant hits . . . lowest E-value is 1.6
Spacer Forty-Three: ACTCCGGGTACACTGGTGGCGATGCTCTACTCGCC
No significant hits . . . lowest E-value is 1.3
Spacer Forty-Four: ACACGTTTCTTTTTTTCAGGAGCCATCACTCACTC
No significant hits . . . lowest E-value is 1.3
Other things i want to do:
compare our species spacers with themselves look to see if these blast results show hits to any other halophiles look at spacer length and number between our species and others?? any known viruses that infect halophiles??
