Difference between revisions of "Pouring an Agarose Gel"

Revision as of 13:12, 30 June 2011

///Pouring an Agarose Gel///

Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.

Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
Add 35 mL of TAE buffer
Microwave for 1 minute
Swirl and microwave for 10 seconds
Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
Swirl to mix and pour into gel tray with comb

@@ Line 1: / Line 1: @@
-•	0.35 grams of Agarose
+///Pouring an Agarose Gel///
-•	35 mL of TAE Buffer
-•	Add both to an Erlenmeyer Flask and microwave for 1 minute
+#Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.
-•	Swirl and microwave for 10 seconds
-•	Let flask cool enough to touch, add 5 µL of Ethidium Bromide (WEAR GLOVES)
+# Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
-•	Swirl to mix and pour into gel tray
+# Add 35 mL of TAE buffer
+# Microwave for 1 minute
+# Swirl and microwave for 10 seconds
+# Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
+# Swirl to mix and pour into gel tray with comb