Difference between revisions of "Prepare PCR product for sequencing"
From GcatWiki
Macampbell (talk | contribs) (Created page with 'You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product. # Pipet 5 µL of clean PCR product into the bar…') |
Macampbell (talk | contribs) |
||
| Line 1: | Line 1: | ||
You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product. | You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product. | ||
| − | # Pipet | + | # Pipet 8 µL of clean PCR product into the bar coded tube. |
| − | # Pipet | + | # Pipet 4 µL of sequencing primer into the same bar coded tube. |
# Place the small cap back onto the bar coded sequencing tube. | # Place the small cap back onto the bar coded sequencing tube. | ||
# Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab. | # Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab. | ||
Revision as of 14:56, 7 August 2012
You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.
- Pipet 8 µL of clean PCR product into the bar coded tube.
- Pipet 4 µL of sequencing primer into the same bar coded tube.
- Place the small cap back onto the bar coded sequencing tube.
- Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
