Difference between revisions of "Prepare PCR product for sequencing"
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# Pipet 8 µL of PCR product into the bar coded tube. | # Pipet 8 µL of PCR product into the bar coded tube. | ||
| − | # Pipet 4 µL of sequencing primer into the same bar coded tube. | + | # Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube. |
# Place the small cap back onto the bar coded sequencing tube. | # Place the small cap back onto the bar coded sequencing tube. | ||
# Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab. | # Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab. | ||
# The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon]. | # The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon]. | ||
Revision as of 15:51, 9 October 2012
You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.
- Pipet 8 µL of PCR product into the bar coded tube.
- Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
- Place the small cap back onto the bar coded sequencing tube.
- Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
- The information will be submitted to the sequencing company MWG Operon.
