Difference between revisions of "Prepare PCR product for sequencing"

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You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.  
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You have amplified your TAS2R38 alleles for sequencing. Now you need to mix the sequencing primer with your PCR product.  
  
 
# Pipet 8 µL of PCR product into the bar coded tube.
 
# Pipet 8 µL of PCR product into the bar coded tube.

Revision as of 03:03, 11 October 2012

You have amplified your TAS2R38 alleles for sequencing. Now you need to mix the sequencing primer with your PCR product.

  1. Pipet 8 µL of PCR product into the bar coded tube.
  2. Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
  3. Place the small cap back onto the bar coded sequencing tube.
  4. Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
  5. The information will be submitted to the sequencing company MWG Operon.
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