Difference between revisions of "Davidson Projects with Updates"

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(Building LasR receiver '''TESTER''' cell)
Line 12: Line 12:
  
 
== Building LasR receiver '''TESTER''' cell ==
 
== Building LasR receiver '''TESTER''' cell ==
Ligatiion 1A S03156 + R0011
+
Ligatiion 1A S03156 + R0011<br>
Ligation 1B R0079 + E0240
+
Ligation 1B R0079 + E0240<br>
Ligation 1C B0015 + R0079
+
Ligation 1C B0015 + R0079<br>
 
+
<br>
Ligation 2: Successful Ligation 1A + uccessful Ligation 1B
+
Ligation 2: Successful Ligation 1A + uccessful Ligation 1B<br>
Ligation 3: Successful Ligation 2 + R0011
+
Ligation 3: Successful Ligation 2 + R0011<br>
  
 
== Testing Crosstalk between Lux and Las ==
 
== Testing Crosstalk between Lux and Las ==

Revision as of 21:21, 23 June 2008

Building LuxI sender TESTER cell

R0011 (verified) + F1610 (not right part from registry)
Going after S03608 (pLac_RBS_LuxI) from 2008 paper registry :-(
If we get this and verify, we are done with this. If not, we are in need of LuxI part.

Building LuxR receiver TESTER cell

Part K09100 has been built.
Ready to test.

Building LasI sender TESTER cell

R0011+ S03154.

Building LasR receiver TESTER cell

Ligatiion 1A S03156 + R0011
Ligation 1B R0079 + E0240
Ligation 1C B0015 + R0079

Ligation 2: Successful Ligation 1A + uccessful Ligation 1B
Ligation 3: Successful Ligation 2 + R0011

Testing Crosstalk between Lux and Las

Testing AND gate

Building XOR Gate

Testing XOR Gate

AND gate using pTetLac promoter